{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T04:16:22Z","timestamp":1783656982562,"version":"3.55.0"},"reference-count":65,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"ABSTRACT<\/jats:title>\n \n \n Aeromonas hydrophila<\/jats:italic>\n <\/jats:styled-content>\n and\n \n Aeromonas caviae<\/jats:italic>\n <\/jats:styled-content>\n are two economically significant pathogenic bacteria in aquaculture and are the main causes of bacterial septicemia in fish. Controlling disease caused by these two bacterial pathogens is becoming a greater challenge due to increases in the antibiotic resistance of bacteria. Here, we developed and applied novel DNA vaccines carrying antigen genes for ovalbumin (OVA) and the outer membrane protein (OMP) of\n \n A. hydrophila<\/jats:italic>\n <\/jats:styled-content>\n to evaluate their immunoprotective effects against these two\n Aeromonas<\/jats:italic>\n species and promote the green development of the aquaculture industry.\n \n Sinocyclocheilus grahami<\/jats:italic>\n <\/jats:styled-content>\n (\n \n S. grahami<\/jats:italic>\n <\/jats:styled-content>\n ) and\n \n Oreochromis niloticus<\/jats:italic>\n <\/jats:styled-content>\n (\n \n O. niloticus<\/jats:italic>\n <\/jats:styled-content>\n ) were subjected to oral vaccination treatment for a total of 21\u2009days. Seven days after being injected with 100\u2009\u03bcL of 1\u2009\u00d7\u200910\n 9<\/jats:sup>\n \u2009CFU\/mL of\n \n A. hydrophila<\/jats:italic>\n <\/jats:styled-content>\n or\n \n A. caviae<\/jats:italic>\n <\/jats:styled-content>\n , significant decreases were observed in the following non\u2010specific immune indicators: superoxide dismutase (SOD), complement C3 (C3), lysozyme (LZM) and immunoglobulin M (IgM). However, levels of SOD, C3, LZM and IgM remained stable in fish serum following administration of the OVA, OMP and OVA\u2010OMP vaccines. Histopathological analysis indicated that oral vaccination with the OVA vaccine could alleviate the splenic lesions caused by\n \n A. hydrophila<\/jats:italic>\n <\/jats:styled-content>\n and\n \n A. caviae<\/jats:italic>\n <\/jats:styled-content>\n in\n \n S. grahami<\/jats:italic>\n <\/jats:styled-content>\n . After challenge with\n \n A. hydrophila<\/jats:italic>\n <\/jats:styled-content>\n , the immune protection rates of the OVA, OMP and OVA\u2010OMP vaccines in\n \n S. grahami<\/jats:italic>\n <\/jats:styled-content>\n were 92.56%, 88.89% and 73.33%, respectively, and the relative percent survival (RPS) of\n \n O. niloticus<\/jats:italic>\n <\/jats:styled-content>\n was 75%, 83.38% and 50%, respectively. Following challenge with\n \n A. caviae<\/jats:italic>\n <\/jats:styled-content>\n , the RPS values for\n \n S. grahami<\/jats:italic>\n <\/jats:styled-content>\n vaccinated with the OVA, OMP and OVA\u2010OMP vaccines were 100%, 86.96% and 95.70%, respectively, and the RPS values for\n \n O. niloticus<\/jats:italic>\n <\/jats:styled-content>\n were 92.27%, 96.19% and 92.27%, respectively. In conclusion, oral administration of the OVA, OMP and OVA\u2010OMP DNA vaccines significantly enhanced both non\u2010specific and specific immune responses, providing strong protection against\n \n A. hydrophila<\/jats:italic>\n <\/jats:styled-content>\n and\n \n A. caviae<\/jats:italic>\n <\/jats:styled-content>\n infections in\n \n S. grahami<\/jats:italic>\n <\/jats:styled-content>\n and\n \n O. niloticus<\/jats:italic>\n <\/jats:styled-content>\n . Future work should focus on optimizing vaccine stability (e.g., improving the OVA\u2010OMP fusion construct), expanding coverage to other economically important and endangered fish species and clarifying long\u2010term immune memory mechanisms to support sustainable aquaculture development.\n <\/jats:p>","DOI":"10.1111\/jfd.70241","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T03:45:31Z","timestamp":1783655131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of the Immunological Effects of\n DNA<\/scp>\n Vaccines Carrying Antigen Genes for\n OVA<\/scp>\n ,\n OMP<\/scp>\n and\n OVA<\/scp>\n \u2010\n OMP<\/scp>\n Against\n Aeromonas hydrophila<\/i>\n and\n Aeromonas caviae<\/i>\n Infections in\n Sinocyclocheilus grahami<\/i>\n and\n Oreochromis niloticus<\/i>"],"prefix":"10.1111","author":[{"given":"Long","family":"Zhu","sequence":"first","affiliation":[{"name":"Yunnan Key Laboratory of Plateau Fish Breeding\/Yunnan Engineering Research Center for Plateau Lake Health and Restoration Kunming Institute of Zoology, Chinese Academy of Sciences, University of Chinese Academy of Sciences Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8527-9953","authenticated-orcid":false,"given":"Guowen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Laboratory of Peptides of Chinese Academy of Sciences Key Laboratory of Bioactive Peptides of Yunnan Province and State Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, the Chinese Academy of Sciences Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Rural Affairs of Yunnan Province Yunnan Academy of Fishery Sciences Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Rural Affairs of Yunnan Province Yunnan Aquatic Product Technology Extension Station Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouyou","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Rural Affairs of Yunnan Province Yuanjiang County Aquatic Product Technology Extension Station Yuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Rural Affairs of Yunnan Province Yuanjiang County Aquatic Product Technology Extension Station Yuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Rural Affairs of Yunnan Province Yuanjiang County Aquatic Product Technology Extension Station Yuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinrong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Rural Affairs of Yunnan Province Yuanjiang County Aquatic Product Technology Extension Station Yuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lincong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Rural Affairs of Yunnan Province Yuanjiang County Aquatic Product Technology Extension Station Yuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7330-7596","authenticated-orcid":false,"given":"Xiaofu","family":"Pan","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Plateau Fish Breeding\/Yunnan Engineering Research Center for Plateau Lake Health and Restoration Kunming Institute of Zoology, Chinese Academy of Sciences, University of Chinese Academy of Sciences Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2621-5782","authenticated-orcid":false,"given":"Wenhui","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Laboratory of Peptides of Chinese Academy of Sciences Key Laboratory of Bioactive Peptides of Yunnan Province and State Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, the Chinese Academy of Sciences Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/lam.12484","article-title":"Comparative Analysis of Virulence Genes, Antibiotic Resistance and gyrB\u2010Based Phylogeny of Motile Aeromonas Species Isolates From Nile Tilapia and Domestic Fowl","volume":"61","author":"Abu\u2010Elala N.","year":"2015","journal-title":"Letters in Applied Microbiology"},{"key":"e_1_2_12_3_1","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.fsi.2019.04.066","article-title":"Progress, Challenges and Opportunities in Fish Vaccine Development","volume":"90","author":"Adams A.","year":"2019","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1002\/etc.2144","article-title":"A Method to Predict and Understand Fish Survival Under Dynamic Chemical 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F.","year":"2024","journal-title":"BMC Veterinary Research"},{"key":"e_1_2_12_6_1","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1177\/1040638717708584","article-title":"Lesions Caused by Virulent \n Aeromonas hydrophila\n in Farmed Catfish (\n Ictalurus punctatus\n and \n I. punctatus\n \u00d7 \n I. furcatus\n ) in Mississippi","volume":"29","author":"Baumgartner W. A.","year":"2017","journal-title":"Journal of Veterinary Diagnostic Investigation"},{"key":"e_1_2_12_7_1","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.1007\/s42770-025-01715-9","article-title":"Tilapia Lake Virus (TiLV), \n Aeromonas caviae\n and Aquatic Environmental Variation\u2014The Triad Stressors Lead Mass Mortality in \n Oreochromis niloticus\n in Wetlands of West Bengal, India","volume":"56","author":"Bera A. K.","year":"2025","journal-title":"Brazilian Journal of Microbiology"},{"key":"e_1_2_12_8_1","first-page":"105","article-title":"Growth and Mortality of the Fish \n Haemulon aurolineatum\n (Teleostei: Haemulidae) From Southwest of Margarita Island, Venezuela","volume":"57","author":"Bravo E.","year":"2009","journal-title":"Revista de Biolog\u00eda Tropical"},{"key":"e_1_2_12_9_1","doi-asserted-by":"crossref","DOI":"10.1038\/srep15458","article-title":"Disparate Developmental Patterns of Immune Responses to Bacterial and Viral Infections in Fish","volume":"5","author":"Castro R.","year":"2015","journal-title":"Scientific Reports"},{"key":"e_1_2_12_10_1","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.fsi.2020.03.064","article-title":"Immune Sensing of DNA and Strategies for Fish DNA Vaccine Development","volume":"101","author":"Chang C. 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A.","year":"2014","journal-title":"Journal of Fish Diseases"},{"key":"e_1_2_12_13_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0245207","article-title":"Poria Cocos Polysaccharide Induced Th1\u2010Type Immune Responses to Ovalbumin in Mice","volume":"16","author":"Dong X.","year":"2021","journal-title":"PLoS One"},{"key":"e_1_2_12_14_1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1112941","article-title":"The Mobile Gene Cassette Carrying Tetracycline Resistance Genes in \n Aeromonas veronii\n Strain Ah5S\u201024 Isolated From Catfish Pond Sediments Shows Similarity With a Cassette Found in Other Environmental and Foodborne Bacteria","volume":"14","author":"Dubey S.","year":"2023","journal-title":"Frontiers in Microbiology"},{"key":"e_1_2_12_15_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0034-5288(02)00032-2","article-title":"The Application of Nucleic Acid Vaccines in Veterinary Medicine","volume":"73","author":"Dunham S. 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R.","year":"2010","journal-title":"Cell Research"},{"key":"e_1_2_12_17_1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1264\/jsme2.ME12009","article-title":"Aeromonas hydrophila\n Subsp. dhakensis Isolated From Feces, Water and Fish in Mediterranean Spain","volume":"27","author":"Esteve C.","year":"2012","journal-title":"Microbes and Environments"},{"key":"e_1_2_12_18_1","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms8010129","article-title":"An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity","volume":"8","author":"Fern\u00e1ndez\u2010Bravo A.","year":"2020","journal-title":"Microorganisms"},{"key":"e_1_2_12_19_1","doi-asserted-by":"crossref","DOI":"10.3390\/antibiotics10121534","article-title":"Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic","volume":"10","author":"Ferraboschi P.","year":"2021","journal-title":"Antibiotics"},{"key":"e_1_2_12_20_1","article-title":"Body Size and Mortality Rates in Coral Reef Fishes: A Three\u2010Phase Relationship","volume":"283","author":"Goatley C. 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Biological Sciences"},{"key":"e_1_2_12_21_1","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1016\/j.fsi.2018.10.065","article-title":"Oral Yeast\u2010Based DNA Vaccine Confers Effective Protection From \n Aeromonas hydrophila\n Infection on \n Carassius auratus","volume":"84","author":"Han B.","year":"2019","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_12_22_1","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.fsi.2020.01.006","article-title":"Ghrelin Modulates the Immune Response and Increases Resistance to \n Aeromonas hydrophila\n Infection in Hybrid Tilapia","volume":"98","author":"Han Z.","year":"2020","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_12_23_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.fsi.2021.03.019","article-title":"Oral Vaccination of Nile Tilapia (\n Oreochromis niloticus\n ) Against Francisellosis Elevates Specific Antibody Titres in Serum and Mucus","volume":"113","author":"Hoare R.","year":"2021","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_12_24_1","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.1042977","article-title":"Identification of \n Shewanella putrefaciens\n as a Novel Pathogen of the Largemouth Bass (\n Micropterus salmoides\n ) and Histopathological Analysis of Diseased Fish","volume":"12","author":"Jiang X.","year":"2022","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"e_1_2_12_25_1","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1046\/j.1365-2567.1999.00771.x","article-title":"Mammalian Granulocyte\u2010Macrophage Colony\u2010Stimulating Factor and Some CpG Motifs Have an Effect on the Immunogenicity of DNA and Subunit Vaccines in Fish","volume":"96","author":"Kanellos T. 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K.","year":"2023","journal-title":"BMC Veterinary Research"},{"key":"e_1_2_12_27_1","doi-asserted-by":"crossref","first-page":"33768","DOI":"10.1007\/s11356-018-3232-3","article-title":"Studies on the Growth and Mortality of Indian River Shad, \n Gudusia chapra\n (Hamilton, 1822) From Panchet Reservoir, India","volume":"25","author":"Kumari S.","year":"2018","journal-title":"Environmental Science and Pollution Research International"},{"key":"e_1_2_12_28_1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms17010040","article-title":"Vaccination of Silver Sea Bream (Sparus sarba) Against \n Vibrio alginolyticus\n : Protective Evaluation of Different Vaccinating Modalities","volume":"17","author":"Li J.","year":"2015","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_12_29_1","doi-asserted-by":"crossref","DOI":"10.3390\/antiox11050806","article-title":"An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides After \n Aeromonas hydrophila\n Infection in Grass Carp (\n Ctenopharyngodon idella\n )","volume":"11","author":"Lu Z. Y.","year":"2022","journal-title":"Antioxidants"},{"key":"e_1_2_12_30_1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fsi.2004.09.006","article-title":"Innate Immunity of Fish (Overview)","volume":"20","author":"Magnad\u00f3ttir B.","year":"2006","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_12_31_1","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-015-0241-5","article-title":"Detoxifying \n Escherichia coli\n for Endotoxin\u2010Free Production of Recombinant Proteins","volume":"14","author":"Mamat U.","year":"2015","journal-title":"Microbial Cell Factories"},{"key":"e_1_2_12_32_1","doi-asserted-by":"crossref","DOI":"10.1038\/s43856-025-00943-2","article-title":"Determinants of Antibody Levels and Protection Against Omicron BQ.1\/XBB Breakthrough Infection","volume":"5","author":"Mart\u00edn P\u00e9rez C.","year":"2025","journal-title":"Communications Medicine"},{"key":"e_1_2_12_33_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/1097-0061(20010130)18:2<173::AID-YEA663>3.0.CO;2-F","article-title":"Targeted Gene Deletion in Zygosaccharomyces bailii","volume":"18","author":"Mollapour M.","year":"2001","journal-title":"Yeast"},{"key":"e_1_2_12_34_1","doi-asserted-by":"crossref","DOI":"10.3390\/ani11010125","article-title":"Fish Pathology Research and Diagnosis in Aquaculture of Farmed Fish; a Proteomics Perspective","volume":"11","author":"Moreira M.","year":"2021","journal-title":"Animals"},{"key":"e_1_2_12_35_1","doi-asserted-by":"crossref","DOI":"10.1111\/jfd.13910","article-title":"Influence of Probiotic and Prebiotic Supplementation on Intestinal Microbiota and Resistance to \n Edwardsiella ictaluri\n Infection in Channel Catfish (\n Ictalurus punctatus\n ) Following Florfenicol Administration","volume":"47","author":"Older C. E.","year":"2024","journal-title":"Journal of Fish Diseases"},{"key":"e_1_2_12_36_1","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.vetmic.2013.02.020","article-title":"Identification, Virulence, and Mass Spectrometry of Toxic ECP Fractions of West Alabama Isolates of \n Aeromonas hydrophila\n Obtained From a 2010 Disease Outbreak","volume":"164","author":"Pridgeon J. W.","year":"2013","journal-title":"Veterinary Microbiology"},{"key":"e_1_2_12_37_1","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1128\/AEM.68.5.2095-2100.2002","article-title":"Auxotrophic Yeast Strains in Fundamental and Applied Research","volume":"68","author":"Pronk J. T.","year":"2002","journal-title":"Applied and Environmental Microbiology"},{"key":"e_1_2_12_38_1","doi-asserted-by":"crossref","first-page":"250","DOI":"10.14202\/vetworld.2023.250-257","article-title":"Aeromonas hydrophila\n Induction Method in Adult Zebrafish (\n Danio rerio\n ) as Animal Infection Models","volume":"16","author":"Qosimah D.","year":"2023","journal-title":"Veterinary World"},{"key":"e_1_2_12_39_1","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.fsi.2021.09.035","article-title":"Supplementation of Zebrafish (\n Danio rerio\n ) Diet Using a Short Antimicrobial Peptide: Evaluation of Growth Performance, Immunomodulatory Function, Antioxidant Activity, and Disease Resistance","volume":"119","author":"Rashidian G.","year":"2021","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_12_40_1","doi-asserted-by":"crossref","DOI":"10.1002\/vms3.70276","article-title":"Disease Prevalence, Usage of Aquaculture Medicinal Products and Their Sustainable Alternatives in Freshwater Aquaculture of North\u2010Central Bangladesh","volume":"11","author":"Rasul M. N.","year":"2025","journal-title":"Veterinary Medicine and Science"},{"key":"e_1_2_12_41_1","doi-asserted-by":"crossref","first-page":"134","DOI":"10.2144\/99261rr02","article-title":"General Method for Plasmid Construction Using Homologous Recombination","volume":"26","author":"Raymond C. K.","year":"1999","journal-title":"BioTechniques"},{"key":"e_1_2_12_42_1","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.3390\/v5061395","article-title":"Regulation of Human Cytomegalovirus Transcription in Latency: Beyond the Major Immediate\u2010Early Promoter","volume":"5","author":"Reeves M.","year":"2013","journal-title":"Viruses"},{"key":"e_1_2_12_43_1","doi-asserted-by":"crossref","DOI":"10.3390\/ani13213338","article-title":"Bivalent Vaccine Against \n Streptococcus agalactiae\n and \n Aeromonas hydrophila\n in Nile Tilapia (\n Oreochromis niloticus\n ): A Laboratory\u2010Phase and Large\u2010Scale Study","volume":"13","author":"Rivas A. V.","year":"2023","journal-title":"Animals"},{"key":"e_1_2_12_44_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.738436","article-title":"Interaction Between Weight and Strain of Nile Tilapia Broodstocks in Relation to Growth and Reproductive Performance","volume":"559","author":"Rossato K. A.","year":"2022","journal-title":"Aquaculture"},{"key":"e_1_2_12_45_1","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1016\/j.dci.2011.11.009","article-title":"Mucosal Immunoglobulins and B Cells of Teleost Fish","volume":"35","author":"Salinas I.","year":"2011","journal-title":"Developmental & Comparative Immunology"},{"key":"e_1_2_12_46_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2023.e14088","article-title":"A Review on Pathogenicity of \n Aeromonas hydrophila\n and Their Mitigation Through Medicinal Herbs in Aquaculture","volume":"9","author":"Semwal A.","year":"2023","journal-title":"Heliyon"},{"key":"e_1_2_12_47_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/aah.10099","article-title":"Vaccination of Tilapia Against Motile Aeromonas Septicemia: A Review","volume":"32","author":"Shirajum Monir M.","year":"2020","journal-title":"Journal of Aquatic Animal Health"},{"key":"e_1_2_12_48_1","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/foab018","article-title":"Yeasts as a Promising Delivery Platform for DNA and RNA Vaccines","volume":"21","author":"Silva A. 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