{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T15:06:02Z","timestamp":1784214362403,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP25K02098"],"award-info":[{"award-number":["JP25K02098"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Lactococcosis is a major bacterial disease that affects Japanese\n                    <jats:italic>Seriola<\/jats:italic>\n                    aquaculture; however, its molecular epidemiology remains poorly understood. This study characterized 62\n                    <jats:italic>Lactococcus<\/jats:italic>\n                    isolates recovered from Japanese amberjack, greater amberjack and striped jack between 2016 and 2025 using multiplex polymerase chain reaction, multilocus sequence typing (MLST) and erythromycin susceptibility testing. An epidemiological shift was observed from the dominant ST17\u2010serotype I lineage (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lactococcus garvieae<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) to ST56\u2010 and ST115\u2010serotype II lineages (\n                    <jats:italic>Lactococcus formosensis<\/jats:italic>\n                    ), with the emergence of ST95\u2010serotype III (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. garvieae<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). MLST demonstrated an association between sequence type, serotype and erythromycin susceptibility. ST56 remained susceptible to erythromycin, whereas ST115 showed erythromycin resistance, harboured the\n                    <jats:italic>erm<\/jats:italic>\n                    (B) gene and represented a previously unreported lineage. Furthermore, goeBURST and phylogenetic analyses revealed that ST56 and ST115 belonged to the same clonal complex and differed at a single locus, indicating recent diversification within the endemic serotype II lineage. Conversely, ST95 formed a singleton lineage, consistent with a distinct evolutionary origin. ST115 detection at multiple aquaculture sites suggests dissemination through fish movement, including imported and translocated juveniles. These findings indicate changes in\n                    <jats:italic>Lactococcus<\/jats:italic>\n                    population structure in Japanese aquaculture and highlight the need for molecular surveillance, improved vaccines and biosecurity to limit the spread of emerging antimicrobial\u2010resistant lineages.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70250","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T14:40:36Z","timestamp":1784212836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multilocus Sequence Typing\u2010Based Molecular Epidemiology of\n                    <i>Lactococcus garvieae<\/i>\n                    and\n                    <i>Lactococcus formosensis<\/i>\n                    in Japanese Amberjack, Greater Amberjack and Striped Jack Aquaculture"],"prefix":"10.1111","author":[{"given":"Salif","family":"Maiga","sequence":"first","affiliation":[{"name":"Department of Bioresource Production Science, The United Graduate School of Agricultural Sciences Ehime University  Ehime Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazushi","family":"Sakasai","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Marine Science, Graduate School of Integrated Arts and Science Kochi University  Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azumi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Marine Science, Graduate School of Integrated Arts and Science Kochi University  Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1398-3782","authenticated-orcid":false,"given":"Haruhisa","family":"Fukada","sequence":"additional","affiliation":[{"name":"Department of Bioresource Production Science, The United Graduate School of Agricultural Sciences Ehime University  Ehime Japan"},{"name":"Faculty of Agriculture and Marine Science, Graduate School of Integrated Arts and Science Kochi University  Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2013-5730","authenticated-orcid":false,"given":"Masayuki","family":"Imajoh","sequence":"additional","affiliation":[{"name":"Department of Bioresource Production Science, The United Graduate School of Agricultural Sciences Ehime University  Ehime Japan"},{"name":"Faculty of Agriculture and Marine Science, Graduate School of Integrated Arts and Science Kochi University  Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2013.12.003"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13793"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00890\u201022"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao008171"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.70110"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.59.63"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00541\u201024"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.052811\u20100"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002849900015"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0966\u2010842x(99)01609\u20101"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084796"},{"key":"e_1_2_11_13_1","volume-title":"Diagnostic Manual for Diseases of Cultured Greater Amberjack, Revised Edition","author":"Fish Disease Control Technical Manual Editorial Committee","year":"2018"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2026.105189"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.50.200"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.11233\/aquaculturesci.63.59"},{"key":"e_1_2_11_17_1","first-page":"95","article-title":"BioEdit: A User\u2010Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95\/98\/NT","volume":"41","author":"Hall T. 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