{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T10:29:46Z","timestamp":1794306586520,"version":"4.9.0"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100019065","name":"Tianjin Municipal Science and Technology Program","doi-asserted-by":"publisher","award":["24YDTPJC00330"],"award-info":[{"award-number":["24YDTPJC00330"]}],"id":[{"id":"10.13039\/501100019065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cynoglossus semilaevis<\/jats:italic>\n                    <\/jats:styled-content>\n                    parvovirus (CsPV) is an important pathogen that poses a serious threat to the aquaculture industry. In the absence of vaccines and effective therapeutic drugs, early and sensitive detection of the pathogen is the key measure to prevent and control the outbreaks and epidemics of viral diseases. Currently, the existing detection methods for CsPV are expensive and rely on specialized operations, limiting the application of on\u2010site detection. There is an urgent need for a low\u2010cost, user\u2010friendly detecting platform to prevent fish viral diseases. In this study, we confirmed skin mucus as a target tissue for non\u2010lethal sampling, established a simple proteinase K digestion, 2% CTAB lysis and silica\u2010hydroxylated magnetic beads adsorption method for extracting viral genomic DNA from skin mucus and developed a visual one\u2010pot loop\u2010mediated isothermal amplification (LAMP) detection method with a sensitivity of 4.69\u2009\u00d7\u200910\n                    <jats:sup>1<\/jats:sup>\n                    copies\/\u03bcL. Validation of the detection platform using clinical samples showed 97% sensitivity and 100% specificity. The cost of reagents for per sample was reduced from 16.6 CNY to 6.2 CNY. The low\u2010cost naked\u2010eye on\u2010site detection platform provides technical support for resource\u2010limited aquaculture farms to prevent and control emerging viral diseases in Chinese tongue sole.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70258","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T04:40:52Z","timestamp":1784954452000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Lab to Farm: A Low\u2010Cost Naked\u2010Eye On\u2010Site Detecting Platform for\n                    <i>Cynoglossus semilaevis<\/i>\n                    Parvovirus Based on Magnetic Beads Adsorption and\n                    <scp>LAMP<\/scp>\n                    \u2010\n                    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