{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:58:36Z","timestamp":1785387516646,"version":"3.55.0"},"reference-count":56,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In February 2025, a mortality event of unknown aetiology occurred at a hybrid grouper (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Epinephelus fuscoguttatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2009\u00d7\u2009\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Epinephelus lanceolatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) aquaculture farm in southern Taiwan, with sporadic daily deaths persisting for a month. Four moribund groupers were collected over three sampling intervals for diagnostic investigation. Gross pathology revealed opaque white discoloration with multifocal petechial haemorrhages in the lateral musculature. Gram\u2010stained muscular viscous fluid smears showed abundant Gram\u2010positive, filamentous bacilli (2\u2013100\u2009\u03bcm in length). Histopathology of the skeletal muscle confirmed extensive Zenker's necrosis, with dense bacterial aggregates infiltrating necrotic myofibres. On blood agar at 28\u00b0C, smooth, greyish, \u03b1\u2010haemolytic colonies developed within 16\u201324\u2009h; growth was restricted at 32\u00b0C and absent at 35\u00b0C. Full\u2010length 16S rRNA gene sequencing identified the isolates as\n                    <jats:italic>Erysipelothrix<\/jats:italic>\n                    . High\u2010resolution\n                    <jats:italic>gyrB<\/jats:italic>\n                    \u2010based phylogenetic analysis clustered the isolates nearly identically with the dolphin\u2010derived\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. rhusiopathiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    strain KC\u2010Sb\u2010R1 (CP033601), distinct from swine\u2010derived\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E.\u2009rhusiopathiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    (97.0%),\n                    <jats:italic>E.\u2009piscisicarius<\/jats:italic>\n                    (91.2%) and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E.\u2009tonsillarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    (83.9%). The isolates belonged to\n                    <jats:italic>spa<\/jats:italic>\n                    type B. To our knowledge, this is the first documented case of an\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E.\u2009rhusiopathiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010like infection causing myositis and intramuscular abscesses in grouper aquaculture, highlighting a potentially marine\u2010associated lineage with significant implications for aquatic veterinary medicine and public health.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70262","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:02:23Z","timestamp":1785384143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Identification of\n                    <i>Erysipelothrix<\/i>\n                    sp. in Hybrid Grouper (\n                    <i>Epinephelus fuscoguttatus<\/i>\n                    \u2009\u00d7\u2009\n                    <i>E. lanceolatus<\/i>\n                    ) in Taiwan"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7093-9360","authenticated-orcid":false,"given":"Shang\u2010Ting","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine National Chung Hsing University  Taichung City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Siang","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine National Chung Hsing University  Taichung City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6103-3453","authenticated-orcid":false,"given":"Chia\u2010Yu","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine National Chung Hsing University  Taichung City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.03.006"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010\u2010016\u20100966\u2010z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063871102300126"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12081339"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/pl00006809"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615\u201048\u20109\u2010789"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03609"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.06.049"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13485"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1303235"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/10406387231209035"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12293"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.09.012"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12030409"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/46.4.472"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.12.016"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.5.2066\u20102070.2000"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010016\u20102643\u20100"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2779\u20102785.2002"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.05.012"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.104421"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201082959\u2010x"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.47363\/JCET\/2020(2)115"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2018.1447171"},{"key":"e_1_2_11_26_1","volume-title":"Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: M45","author":"Hindler J. A.","year":"2016"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.736193"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4376\u20104382.1999"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.01.041"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567\u2010015\u20100216\u2010x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.7589\/JWD\u2010D\u201020\u201000177"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63650\u20100"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.774836"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.6.1526\u20101531.1994"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2017.04.012"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.01500\u201010"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00174"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2012.08.004"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13145"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003838"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12673"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01643969"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.2.4.354"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae959"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2010.04746.x"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638719835883"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.15344\/2456\u20104028\/2018\/134"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03332"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4093\u20104098.1999"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00099\u201007"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.10.2038"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102230.2009.03444.x"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.08.012"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.981477"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13020441"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12758"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70262","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:02:24Z","timestamp":1785384144000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":56,"alternative-id":["10.1111\/jfd.70262"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70262","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]},"assertion":[{"value":"2026-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70262"}}