{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T06:59:19Z","timestamp":1785567559194,"version":"3.56.0"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2026GXNSFBA00640146"],"award-info":[{"award-number":["2026GXNSFBA00640146"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["Guike Nong AB2506910047"],"award-info":[{"award-number":["Guike Nong AB2506910047"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Aeromonas hydrophila<\/jats:italic>\n                    <\/jats:styled-content>\n                    is a major pathogen of Motile Aeromonad Septicemia (MAS) in Nile tilapia, with pathogenesis and immunity poorly understood. In early 2026, a &gt;\u200970% mortality outbreak occurred at a Nanning tilapia farm. The dominant strain NN0116 was identified as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. hydrophila<\/jats:italic>\n                    <\/jats:styled-content>\n                    by 16S rRNA and whole\u2010genome ANI analysis. It exhibited high virulence (LD\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20095.2\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    \u2009CFU\/fish). Its 5.37\u2009Mb genome encodes 5065 proteins and contains T2SS, T3SS, T6SS, and pore\u2010forming toxins. Virulence and antimicrobial resistance genes co\u2010localized on genomic islands GI2 and GI7. The strain was resistant to 12 antibiotic classes but susceptible to third\u2010generation cephalosporins and fluoroquinolones. Head kidney transcriptomics at 24\u2009h identified 1066 differentially expressed genes, including 61 immunoglobulin (Ig) genes. KEGG enrichment revealed 43 significant pathways; the top ten were driven by 26 Ig genes. Network analysis of the top 20 pathways identified B cell receptor signalling (ko04662) and COVID\u201019 (ko05171) as central nodes, alongside 10 secondary core pathways, all Ig\u2010driven. Integration of bacterial genomic and host transcriptomic data characterizes hypervirulent\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. hydrophila<\/jats:italic>\n                    <\/jats:styled-content>\n                    NN0116 and demonstrates that Ig\u2010mediated B cell responses are central to host defence, offering targets for vaccines and therapeutics.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70266","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T06:43:02Z","timestamp":1785566582000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathogenicity and Genomic Analysis of Hypervirulent\n                    <i>Aeromonas hydrophila<\/i>\n                    <scp>NN0116<\/scp>\n                    From Nile Tilapia and Head Kidney Transcriptome of Infected Fish Reveals a B\u2010Cell\u2010Dominated Immune Response"],"prefix":"10.1111","author":[{"given":"Fuhao","family":"Geng","sequence":"first","affiliation":[{"name":"Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jifeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zulin","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology Guangxi University  Nanning 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