{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T16:20:30Z","timestamp":1787329230412,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Decapod iridovirus 1 (DIV1) is a highly lethal pathogen that infects decapod crustaceans including\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Litopenaeus vannamei<\/jats:italic>\n                    <\/jats:styled-content>\n                    , causing mass mortality in cultured shrimp and severe economic losses worldwide. The ATPase gene is a highly conserved region within the DIV1 genome, plays a critical role in viral replication and represents an ideal target for molecular diagnostic development. In this study, we established a rapid, sensitive and field\u2010adaptable detection platform for DIV1 by integrating recombinase polymerase amplification (RPA) with the CRISPR\/Cas12a system. RPA enables efficient isothermal amplification of target nucleic acids, achieving exponential enrichment of the target nucleic acids and exerting the function of signal amplification. While the CRISPR\/Cas12a system upon crRNA\u2010guided specific recognition of the amplicon, triggers robust trans\u2010cleavage activity against reporter probes for signal generation and readout. After systematic optimization, the RPA reaction was performed at 38\u00b0C for 10\u2009min and the CRISPR\u2010Cas12a reaction was conducted at 37\u00b0C for 20\u2009min. The integrated two\u2010step workflow completed detection within 40\u2009min, with a limit of detection of 2.3\u2009\u00d7\u200910\n                    <jats:sup>1<\/jats:sup>\n                    copies\/\u03bcL. Specificity evaluation confirmed that the RPA\u2010CRISPR\/Cas12a assay exclusively recognised DIV1 without cross\u2010reaction with other major shrimp pathogens. Further validation using clinical shrimp samples demonstrated stable and reliable performance, supporting its practical utility in aquaculture settings. In conclusion, the established CRISPR\/Cas12a\u2010based detection platform provides a robust technical tool for early warning and on\u2010site rapid screening of DIV1, facilitating timely disease control and risk management in shrimp farming.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70269","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T15:08:07Z","timestamp":1786028887000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Field\u2010Deployable\n                    <scp>RPA<\/scp>\n                    \u2010\n                    <scp>CRISPR<\/scp>\n                    \/Cas12a Detection of Decapod Iridescent Virus 1 in Shrimp"],"prefix":"10.1111","author":[{"given":"Rui","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture Institute of Oceanology, Chinese Academy of Sciences  Qingdao Shandong China"},{"name":"Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center  Qingdao Shandong China"},{"name":"Laboratory of Experimental Marine Biology Institute of Oceanology, Chinese Academy of Sciences  Qingdao Shandong China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center  Qingdao Shandong China"},{"name":"Laboratory of Experimental Marine Biology Institute of Oceanology, Chinese Academy of Sciences  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishuai","family":"Du","sequence":"additional","affiliation":[{"name":"Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center  Qingdao Shandong China"},{"name":"Laboratory of Experimental Marine Biology Institute of Oceanology, Chinese Academy of Sciences  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqin","family":"Tian","sequence":"additional","affiliation":[{"name":"Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center  Qingdao Shandong China"},{"name":"Laboratory of Experimental Marine Biology Institute of Oceanology, Chinese Academy of Sciences  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3037-3767","authenticated-orcid":false,"given":"Tianlong","family":"Qiu","sequence":"additional","affiliation":[{"name":"State Key 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