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Monitoring currently relies on a highly sensitive, but costly and time\u2010consuming qPCR approach. Here, we designed a simplified, rapid and cost\u2010efficient molecular assay for on\u2010site detection of\n                    <jats:italic>Aa<\/jats:italic>\n                    . The novel rapid assay employs a combination of isothermal recombinase polymerase amplification and CRISPR\u2010Cas12a\u2010based detection that can be coupled with fluorescence or lateral flow visualisation. We demonstrate that the novel assay can detect\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. astaci<\/jats:italic>\n                    <\/jats:styled-content>\n                    from tissue and environmental DNA with higher sensitivity than the available qPCR assay and readily distinguishes\n                    <jats:italic>Aa<\/jats:italic>\n                    from its non\u2010pathogenic sister taxon\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. fennicus<\/jats:italic>\n                    <\/jats:styled-content>\n                    . We tested two genomic marker sites for\n                    <jats:italic>Aa<\/jats:italic>\n                    that discriminate closely related oomycetes and incorporate field\u2010deployable lateral flow and fluorescence readouts. Our work will make crayfish plague monitoring broadly accessible to practitioners and non\u2010academic stakeholders as a tool to curb further\n                    <jats:italic>Aa<\/jats:italic>\n                    \u2010driven loss of Europe's imperilled freshwater crustaceans and strengthen preparedness against future incursions of the pathogen in other regions.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70278","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T08:30:53Z","timestamp":1788769853000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A CRISPR\u2010Based Rapid Detection Assay for Crayfish Plague (\n                    <i>Aphanomyces astaci<\/i>\n                    ) From Environmental Samples"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6103-2391","authenticated-orcid":false,"given":"Christopher","family":"Heine","sequence":"first","affiliation":[{"name":"Department of Biogeography Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8731-6312","authenticated-orcid":false,"given":"Amadeus","family":"Plewnia","sequence":"additional","affiliation":[{"name":"Wildlife Health Ghent Ghent University  Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8804-7994","authenticated-orcid":false,"given":"David","family":"Strand","sequence":"additional","affiliation":[{"name":"Norwegian Veterinary Institute  \u00c5s Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6131-6802","authenticated-orcid":false,"given":"Simran","family":"Gupta","sequence":"additional","affiliation":[{"name":"NSW Department of Primary Industries and Regional Development Elizabeth Macarthur Agricultural Institute  Menangle New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5069-8601","authenticated-orcid":false,"given":"Henrik","family":"Krehenwinkel","sequence":"additional","affiliation":[{"name":"Department of Biogeography Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7287-4810","authenticated-orcid":false,"given":"Gus R.","family":"McFarlane","sequence":"additional","affiliation":[{"name":"NSW Department of Primary Industries and Regional Development Elizabeth Macarthur Agricultural Institute  Menangle New South Wales Australia"},{"name":"Sydney School of Veterinary Science University of Sydney  Camden New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.20506\/rst.15.2.943","article-title":"Geographical Spread of Bacterial and Fungal Diseases of Crustaceans","volume":"15","author":"Alderman D.","year":"1996","journal-title":"Revue Scientifique et Technique de l'OIE"},{"key":"e_1_2_10_3_1","unstructured":"Austin C. 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