{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T07:18:43Z","timestamp":1788333523302,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Spring viremia of carp virus (SVCV), that can infect cyprinoid and koi causing an acute hemorrhagic symptom, with a feature of widespread epidemic, which poses a severe threat to the global aquaculture industry. Given the absence of specific effective medicine or vaccines, the early, accurate diagnosis is critical in the disease prevention. Many SVCV detection methods have been established, but most require specialized equipment and skilled operators, which limits their field application. In this study, we established a recombinase\u2010mediated isothermal amplification (RAA) in conjunction with the CRISPR\/Cas12a system targeting the screened conserved region of the\n                    <jats:italic>G<\/jats:italic>\n                    gene in the genome to identify SVCV. The optimal combination of the primer pairs and crRNA was screened via the fluorescence intensity. In addition, the test exhibited high sensitivity and specificity, which could detect at the limit of 1.243\u2009\u00d7\u200910\n                    <jats:sup>1<\/jats:sup>\n                    copies\/\u03bcL and show no cross\u2010reactivity with other common freshwater fish pathogens. Furthermore, the result could be visible by excitation of fluorophores by blue light at 39\u00b0C within 30\u2009min. The integrated method combines the advantages of RAA and CRISPR\u2010based signal transduction, offering a diagnosis project in resource\u2010limited aquaculture environments and a field\u2010applicable early warning of SVCV.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70284","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T07:06:52Z","timestamp":1788332812000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Detection of Spring Viremia of Carp Virus (\n                    <scp>SVCV<\/scp>\n                    ) Using\n                    <scp>RAA<\/scp>\n                    \u2010\n                    <scp>CRISPR<\/scp>\n                    \/Cas12a"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7651-4771","authenticated-orcid":false,"given":"Xiaoyang","family":"Li","sequence":"first","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture  Zhanjiang China"},{"name":"College of Animal Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture  Zhanjiang China"},{"name":"College of Animal Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2939-2779","authenticated-orcid":false,"given":"Yingying","family":"Li","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1397-3058","authenticated-orcid":false,"given":"Yingying","family":"Wang","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zheng","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5574-008X","authenticated-orcid":false,"given":"Defeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ren","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0242-0973","authenticated-orcid":false,"given":"Xubing","family":"Mo","sequence":"additional","affiliation":[{"name":"Pearl River Fisheries Research Institute, Key Labortory of Fishery Drug Development (Ministry of Agriculture and Rural Affair), Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture Chinese Academy of Fishery Science  Guangzhou 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