{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:08:16Z","timestamp":1788851296043,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD2401403"],"award-info":[{"award-number":["2024YFD2401403"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100031919","name":"Southern Marine Science and Engineering Guangdong Laboratory","doi-asserted-by":"publisher","award":["SML2024SP003"],"award-info":[{"award-number":["SML2024SP003"]}],"id":[{"id":"10.13039\/100031919","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mycobacterium marinum<\/jats:italic>\n                    <\/jats:styled-content>\n                    is one of the most common atypical mycobacteria causing opportunistic infections in humans. It is considered to be one of the most important fish pathogens and is associated with a variety of diseases in freshwater and marine fish. In this study, a rapid detection method for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. marinum<\/jats:italic>\n                    <\/jats:styled-content>\n                    was established based on the Recombinase Polymerase Amplification (RPA) technology and the CRISPR\/Cas12a trans\u2010cut principle. By designing and screening RPA primers and crRNA, the RPA amplification system and CRISPR\/Cas12a detection system were optimized, and an RPA\u2010CRISPR\/Cas12a detection system with high sensitivity and specificity was successfully constructed. The LOD\n                    <jats:sub>95<\/jats:sub>\n                    of this detection method was estimated to be 5.7 copies\/\u03bcL (95% CI: 2.4\u201315.5 copies\/\u03bcL). The RPA\u2010CRISPR assay showed perfect agreement with qPCR, with 100% sensitivity (95% CI: 76.8%\u2013100%) and 100% specificity (95% CI: 73.5%\u2013100%). The RPA\u2010CRISPR\/Cas12a\u2010\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. marinum<\/jats:italic>\n                    <\/jats:styled-content>\n                    detection method established in this study has the advantages of simple operation and visualization of results. It provides an effective tool for rapid on\u2010site detection of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. marinum<\/jats:italic>\n                    <\/jats:styled-content>\n                    and is expected to play an essential role in the monitoring and prevention of aquaculture diseases.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70286","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:28:06Z","timestamp":1788848886000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Rapid\n                    <scp>RPA<\/scp>\n                    \u2010\n                    <scp>CRISPR<\/scp>\n                    \/Cas12a Approach for Detecting\n                    <scp>\n                      <i>Mycobacterium marinum<\/i>\n                    <\/scp>"],"prefix":"10.1111","author":[{"given":"Guangda","family":"Yan","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajian","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangdong Animal Disease Control Center  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8933-5365","authenticated-orcid":false,"given":"Yiqin","family":"Deng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixun","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Guangzhou Guangdong China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory  Zhuhai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4189-7788","authenticated-orcid":false,"given":"Hongling","family":"Ma","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Feng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.TNMI7-0038-2016"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.75"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26146846"},{"key":"e_1_2_11_5_1","first-page":"e1804","article-title":"Harnessing the Synergy of CRISPR\u2010Cas12 With Isothermal Amplification for Precise and Rapid Detection of Tuberculosis: A Systematic Review","volume":"12","author":"Bintoro B. 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