{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T09:44:13Z","timestamp":1791971053933,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/PR59530\/BSA\/33\/564\/2025"],"award-info":[{"award-number":["BT\/PR59530\/BSA\/33\/564\/2025"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/PR41899\/AAQ\/3\/1006\/2021"],"award-info":[{"award-number":["BT\/PR41899\/AAQ\/3\/1006\/2021"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to explore the widespread occurrence of carp edema virus (CEV) in ornamental koi carp available at ornamental shops in Kolathur, Tamil Nadu. The affected koi carps showed various symptoms, such as ulcers, loss of scales, body and fin haemorrhage, fin necrosis, and gill hyperplasia. No known fish cell lines have been reported for the continuous culturing of CEV. To address this constraint, gill (ICG) and heart (SICH) cell lines derived from catla fish were used for the continuous in\u00a0vitro cultivation of CEV. Virus\u2010specific cytopathic effects (CPE) were similarly observed in all 12 consecutive passages in the infected cell lines. Furthermore, the in\u00a0vitro replication efficacy of CEV in ICG and SICH cell lines was confirmed by PCR, and the viral copies were quantified using quantitative PCR (qPCR) at different passages. Initially, the levels of CEV copies were very low; however, they peaked by the 12th passage, indicating adaptation to the ICG and SICH cell lines. In addition, to study the virulence of the isolated CEV, filtrate from the pooled organ tissues of fish, as well as the supernatant of ICG culture of CEV infection, was injected into koi carp, and symptoms were observed and mortality was recorded. PCR and qPCR analyses were conducted to assess tissue tropism in various tissues experimentally CEV\u2010infected koi carp. Based on the PCR and qPCR results, gill tissue was identified as the primary target organ for CEV infection. This study proves that CEV replication in fish cell lines supports future vaccine development.<\/jats:p>","DOI":"10.1111\/jfd.70305","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:44:29Z","timestamp":1791967469000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Screening and Cell Cultured\u2013Based Isolation of Carp Edema Virus in Koi Carp (\n                    <i>Cyprinus carpio<\/i>\n                    Koi) From Aquarium Shops in Tamil Nadu, India"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6574-548X","authenticated-orcid":false,"given":"Gani","family":"Taju","sequence":"first","affiliation":[{"name":"Aquatic Animal Health Laboratory (WOAH Reference Laboratory), PG &amp; Research Department of Zoology C. Abdul Hakeem College (Autonomous)  Melvisharam Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6057-4792","authenticated-orcid":false,"given":"Seepoo","family":"Abdul Majeed","sequence":"additional","affiliation":[{"name":"Aquatic Animal Health Laboratory (WOAH Reference Laboratory), PG &amp; Research Department of Zoology C. Abdul Hakeem College (Autonomous)  Melvisharam Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8662-4956","authenticated-orcid":false,"given":"Siva","family":"Divyalakshmi","sequence":"additional","affiliation":[{"name":"Aquatic Animal Health Laboratory (WOAH Reference Laboratory), PG &amp; Research Department of Zoology C. Abdul Hakeem College (Autonomous)  Melvisharam Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganesan Ajay","family":"Pathra","sequence":"additional","affiliation":[{"name":"Aquatic Animal Health Laboratory (WOAH Reference Laboratory), PG &amp; Research Department of Zoology C. Abdul Hakeem College (Autonomous)  Melvisharam Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6561-6648","authenticated-orcid":false,"given":"Azeez Sait","family":"Sahul Hameed","sequence":"additional","affiliation":[{"name":"Aquatic Animal Health Laboratory (WOAH Reference Laboratory), PG &amp; Research Department of Zoology C. Abdul Hakeem College (Autonomous)  Melvisharam Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13934"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.70014"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12744"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14225"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567\u2010017\u20100416\u20107"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.05.012"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2024.1846\u20101854"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626\u2010024\u201001012\u2010z"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13631"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13474"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u20105\u201013"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.9373"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010021\u201006997"},{"issue":"1","key":"e_1_2_12_15_1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.aquaculture.2006.01.011","article-title":"Establishment and Characterization of India's First Marine Fish Cell Line (SISK) From the Kidney of Sea Bass (\n                     Lates calcarifer\n                  )","volume":"257","author":"Hameed A. 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