{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:42:02Z","timestamp":1792813322190,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Francisella halioticida<\/jats:italic>\n                    <\/jats:styled-content>\n                    has emerged as a significant pathogen of cultured molluscs, causing substantial economic losses due to mass mortalities. The\n                    <jats:italic>Francisella<\/jats:italic>\n                    Pathogenicity Island encodes a Type VI secretion system essential for virulence and intracellular persistence. However, the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F. halioticida<\/jats:italic>\n                    <\/jats:styled-content>\n                    pathogenicity island shows marked sequence divergence from well\u2010characterized\n                    <jats:italic>Francisella<\/jats:italic>\n                    strains, limiting the use of conventional sequence\u2010based annotation. This study performed a comprehensive structure\u2010based annotation of the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F. halioticida<\/jats:italic>\n                    <\/jats:styled-content>\n                    pathogenicity island using AlphaFold3 predictions combined with systematic structural similarity searches. This approach identified five previously uncharacterized FPI components and their respective T6SS homologues. Notably,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F. halioticida<\/jats:italic>\n                    <\/jats:styled-content>\n                    lacks the IglF protein and the N\u2010terminal \u03b1\u2010helix extension of IglG but retains a nearly complete and functionally coherent Type VI secretion system architecture.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70311","type":"journal-article","created":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:28:47Z","timestamp":1792812527000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structure\u2010Based Annotation by\n                    <scp>AlphaFold3<\/scp>\n                    Predictions of the\n                    <i>Francisella halioticida<\/i>\n                    <scp>T6SS<\/scp>\n                    Encoded in the\n                    <i>Francisella<\/i>\n                    Pathogenicity Island"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3622-1468","authenticated-orcid":false,"given":"H\u00e9l\u00e8ne","family":"Bouras","sequence":"first","affiliation":[{"name":"Marine Ecosystems and oRganisms Research Laboratory (MERSEA) University of Caen Normandy  Caen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9258-9876","authenticated-orcid":false,"given":"C\u00e9line","family":"Zatylny\u2010Gaudin","sequence":"additional","affiliation":[{"name":"Marine Ecosystems and oRganisms Research Laboratory (MERSEA) University of Caen Normandy  Caen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3353-4714","authenticated-orcid":false,"given":"Eric","family":"Duchaud","sequence":"additional","affiliation":[{"name":"INRAE, UVSQ, Vim Universit\u00e9 Paris\u2010Saclay  Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,23]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1038\/srep23080","article-title":"Identification of Protein Secretion Systems in Bacterial Genomes","volume":"6","author":"Abby S. 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Bacterium","volume":"89","author":"Kamaishi T.","year":"2010","journal-title":"Diseases of Aquatic Organisms"},{"issue":"2","key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3354\/dao03383","article-title":"Parallel Studies Confirm \n                     Francisella halioticida\n                   Causes Mortality in Yesso Scallops Patinopecten yessoensis","volume":"135","author":"Kawahara M.","year":"2019","journal-title":"Diseases of Aquatic Organisms"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03574"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2025.102984"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.01928","article-title":"Exploring the Diversity Within the Genus Francisella\u2013an Integrated Pan\u2010Genome and Genome\u2010Mining Approach","volume":"11","author":"Kumar R.","year":"2020","journal-title":"Frontiers in Microbiology"},{"issue":"45","key":"e_1_2_9_20_1","doi-asserted-by":"crossref","first-page":"19520","DOI":"10.1073\/pnas.1012931107","article-title":"The \n                     Vibrio cholerae\n                   Type VI Secretion System Displays Antimicrobial Properties","volume":"107","author":"MacIntyre D. 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Nov., Isolated From Farmed Atlantic Cod (\n                     Gadus morhua\n                   L.)","volume":"57","author":"Mikalsen J.","year":"2007","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1409.000"},{"issue":"19","key":"e_1_2_9_25_1","doi-asserted-by":"crossref","first-page":"6430","DOI":"10.1128\/JB.186.19.6430-6436.2004","article-title":"A \n                     Francisella tularensis\n                   Pathogenicity Island Required for Intramacrophage Growth","volume":"186","author":"Nano F. 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