{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:40:45Z","timestamp":1792431645063,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003716","name":"Korea Basic Science Institute","doi-asserted-by":"publisher","award":["RS\u20102026\u201025475931"],"award-info":[{"award-number":["RS\u20102026\u201025475931"]}],"id":[{"id":"10.13039\/501100003716","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"abstract":"ABSTRACT<\/jats:title>\n \n Northern snakehead (\n \n Channa argus<\/jats:italic>\n <\/jats:styled-content>\n ) is a bimodal air\u2010breathing fish in which erythrocyte\u2010associated parameters remain relatively stable during physiological challenges such as aerial exposure. However, whether this characteristic is maintained during systemic infection remains unclear. In this study, temporal host responses were characterized following experimental\n \n Aeromonas salmonicida<\/jats:italic>\n <\/jats:styled-content>\n subsp.\n salmonicida<\/jats:italic>\n infection by integrating haematological, leukocyte morphological, biochemical, and multi\u2010tissue transcriptional analyses. Most erythrocyte\u2010associated indices, including RBC, HGB, HCT, MCV, MCH, and RDW, showed no significant temporal changes, whereas MON, GRA, and PLT exhibited significant responses. Morphologically abnormal leukocytes increased from approximately 7% in healthy fish to 56% at 6\u2009days post\u2010infection and remained above 50% through 8\u2009days, indicating persistent leukocyte\u2010associated cellular alterations during infection. Biochemical responses were also selective, with changes primarily in protein\u2010 and metabolism\u2010associated parameters. Transcriptional responses differed among peripheral blood, head kidney, spleen, and liver, including sustained\n casp7<\/jats:italic>\n suppression in the head kidney and distinct inflammatory and apoptosis\u2010associated responses across tissues. Two\u2010way ANOVA further identified significant tissue \u00d7 time interactions for\n casp8<\/jats:italic>\n ,\n tnf\u03b1<\/jats:italic>\n , and\n casp3<\/jats:italic>\n . Collectively,\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n infection in northern snakehead was characterized by comparatively limited temporal variation in most erythrocyte\u2010associated indices, alongside selective leukocyte, biochemical, and tissue\u2010dependent transcriptional responses. These findings highlight a contrasting blood response characterized by persistent leukocyte alterations despite comparatively limited temporal variation in most erythrocyte\u2010associated indices during bacterial infection in northern snakehead.\n <\/jats:p>","DOI":"10.1111\/jfd.70312","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:21:19Z","timestamp":1792430479000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selective Systemic Responses During\n Aeromonas salmonicida<\/i>\n Subsp.\n Salmonicida<\/i>\n Infection in Northern Snakehead (\n Channa argus<\/i>\n )"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7221-7472","authenticated-orcid":false,"given":"Ishani Bhgaya Vidana","family":"Pallaththarage","sequence":"first","affiliation":[{"name":"Department of Aqualife Medicine Kongju National University Gongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6801-8635","authenticated-orcid":false,"given":"Su\u2010Mi","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Aqualife Medicine Kongju National University Gongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhashini Prasadika","family":"Waidyarathna","sequence":"additional","affiliation":[{"name":"Department of Aqualife Medicine Kongju National University Gongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Jin","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Aqualife Medicine Kongju National University Gongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4435-3164","authenticated-orcid":false,"given":"Min\u2010Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Aqualife Medicine Kongju National University Gongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9521-3804","authenticated-orcid":false,"given":"Myung\u2010Hwa","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Aqualife Medicine Kongju National University Gongju Republic of Korea"},{"name":"Marine and Fisheries Research Institute Kongju National University Gongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Bacterial Fish Pathogens: Diseases of Farmed and Wild Fish","author":"Austin B.","year":"2007"},{"issue":"7","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","DOI":"10.3390\/ani15070965","article-title":"Blood Biochemical Biomarkers in Fish Toxicology\u2014A Review","volume":"15","author":"Bojarski B.","year":"2025","journal-title":"Animals: An Open Access Journal From MDPI"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.fsi.2020.03.022","article-title":"Bacteria\u2010Induced IL\u20101\u03b2 and Its Receptors in Snakehead (\n Channa argus\n ): Evidence for Their Involvement in Antibacterial Innate Immunity","volume":"100","author":"Cui Z. 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The Combined Roles of the Suprabranchial Chamber and Physiological Regulations During Aerial Respiration","volume":"7","author":"Duan T.","year":"2018","journal-title":"Biology Open"},{"issue":"8","key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/S0145-305X(01)00038-6","article-title":"Innate Host Defense Mechanisms of Fish Against Viruses and Bacteria","volume":"25","author":"Ellis A. 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X.","year":"2019","journal-title":"Letters in Applied Microbiology"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1149151","article-title":"Effects of Dietary Tryptophan on the Antioxidant Capacity and Immune Response Associated With TOR and TLRs\/MyD88\/NF\u2010\u03baB Signaling Pathways in Northern Snakehead, \n Channa argus\n (Cantor, 1842)","volume":"14","author":"Zhang X.","year":"2023","journal-title":"Frontiers in Immunology"},{"key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.fsi.2020.05.033","article-title":"Expression of HSP70 Family mRNAs in Albino Northern Snakehead, \n Channa argus\n : Response to Extreme Temperature Stress and Bacterial Infection","volume":"104","author":"Zhou A.","year":"2020","journal-title":"Fish & Shellfish Immunology"},{"issue":"4","key":"e_1_2_10_35_1","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1007\/s12192-017-0869-7","article-title":"HSP60 Expression Profile Under Different Extreme Temperature Stress in Albino Northern Snakehead, \n Channa argus","volume":"23","author":"Zhou A.","year":"2018","journal-title":"Cell Stress and Chaperones"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70312","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70312","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:21:23Z","timestamp":1792430483000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,19]]},"references-count":34,"alternative-id":["10.1111\/jfd.70312"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70312","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,19]]},"assertion":[{"value":"2026-09-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70312"}}