{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T23:08:20Z","timestamp":1768000100773,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,2,26]],"date-time":"2013-02-26T00:00:00Z","timestamp":1361836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"China National Natural Science Foundation Council","doi-asserted-by":"crossref","award":["81001333"],"award-info":[{"award-number":["81001333"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"China National Natural Science Foundation Council","doi-asserted-by":"crossref","award":["81072479"],"award-info":[{"award-number":["81072479"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2013,3]]},"abstract":"Abstract<\/jats:title>Background and Aim<\/jats:title>MicroRNA<\/jats:styled-content>, as an important regulator of gene expression, has been found to be associated with several diseases. MicroRNA<\/jats:styled-content> expression profiles have been identified in several autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus, and multiple sclerosis. However, the expression profile in peripheral blood mononuclear cells (PBMCs<\/jats:styled-content>) from primary biliary cirrhosis (PBC<\/jats:styled-content>) patients and the role of microRNA<\/jats:styled-content> in PBC<\/jats:styled-content> remained unclear. The present study aimed to explore abnormal microRNA<\/jats:styled-content> regulation in PBC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>MicroRNA<\/jats:styled-content> array was performed in PBMCs<\/jats:styled-content> obtained from PBC<\/jats:styled-content> patients versus healthy controls. Then, six of the 17 differentially expressed microRNAs<\/jats:styled-content> were confirmed using quantitative real\u2010time polymerase chain reaction. Based on bioinformatics analysis, we identified the potential biological processes and significant signaling pathways affected by these microRNAs<\/jats:styled-content>, and generated the microRNA<\/jats:styled-content>\u2013gene network.<\/jats:p><\/jats:sec>Results<\/jats:title>According to microRNA<\/jats:styled-content> array, a total of 17 microRNAs<\/jats:styled-content> were found to be differentially expressed. Six microRNAs<\/jats:styled-content> have been validated using quantitative real\u2010time polymerase chain reaction, and the results were consistent with microRNA<\/jats:styled-content> array analysis. The bioinformatics analysis showed that the potential target genes of these microRNAs<\/jats:styled-content> were involved in cell proliferation, cell differentiation, apoptosis, and signal transduction. Similarly, these microRNAs<\/jats:styled-content> also affected endocytosis, mitogen\u2010activated protein kinase signaling pathway, transforming growth factor\u2010\u03b2 signaling pathway, Wnt signaling pathway, calcium signaling pathway, etc.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In the present study, 17 microRNAs<\/jats:styled-content> were identified to be differentially expressed in PBMCs<\/jats:styled-content> from PBC<\/jats:styled-content> patients. Functional bioinformatics analysis demonstrated that prediction genes targeted by these microRNAs<\/jats:styled-content> were involved in multiple biological processes and signaling pathways. The present study offers intriguing new perspectives on the involvement of microRNA<\/jats:styled-content> in PBC<\/jats:styled-content>, but the precise mechanisms need to be validated further.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.12040","type":"journal-article","created":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T18:25:43Z","timestamp":1353608743000},"page":"543-550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Analysis of altered microRNA<\/scp> expression profiles in peripheral blood mononuclear cells from patients with primary biliary cirrhosis"],"prefix":"10.1111","volume":"28","author":[{"given":"Baodong","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Laboratory Diagnostics Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenglou","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics Changzheng Hospital Second Military Medical University Shanghai China"},{"name":"Naval Convalescent Department Hangzhou Sanatorium Nanjing Military District Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zaixing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renqian","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,2,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra043898"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/610504"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.05.005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035366"},{"key":"e_1_2_6_6_1","unstructured":"miRBase the microRNA database.2012. 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