{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T09:52:08Z","timestamp":1785232328368,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T00:00:00Z","timestamp":1358812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["DK62813"],"award-info":[{"award-number":["DK62813"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan","doi-asserted-by":"crossref","award":["22390085"],"award-info":[{"award-number":["22390085"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan","doi-asserted-by":"crossref","award":["22659087"],"award-info":[{"award-number":["22659087"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Discretion of the President, Oita University"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"Abstract<\/jats:title>Background and Aim<\/jats:title>H<\/jats:styled-content>elicobacter pylori<\/jats:italic> cagA<\/jats:italic><\/jats:styled-content> can be classified into mainly two types (E<\/jats:styled-content>ast\u2010A<\/jats:styled-content>sian\u2010type and W<\/jats:styled-content>estern\u2010type cagA<\/jats:italic><\/jats:styled-content>) according to the repeat regions located in the 3\u2032 region. Recent studies showed that the W<\/jats:styled-content>estern\u2010type cagA<\/jats:italic><\/jats:styled-content> in strains from O<\/jats:styled-content>kinawa, J<\/jats:styled-content>apan formed a different cluster (J<\/jats:styled-content>\u2010W<\/jats:styled-content>estern\u2010type cagA<\/jats:italic><\/jats:styled-content> subtype). It has also been reported that J<\/jats:styled-content>\u2010W<\/jats:styled-content>estern\u2010type cagA<\/jats:italic><\/jats:styled-content> possesses a 12\u2010bp insertion located in the 5\u2032 region of cagA<\/jats:italic><\/jats:styled-content> sequence.<\/jats:p><\/jats:sec>Methods<\/jats:title>The prevalence of 12\u2010bp insertion in cagA<\/jats:italic><\/jats:styled-content> in O<\/jats:styled-content>kinawa and the U<\/jats:styled-content>nited S<\/jats:styled-content>tates (U<\/jats:styled-content>.S<\/jats:styled-content>.) was examined by DNA<\/jats:styled-content> sequencing. The primer pair that can detect the 12\u2010bp insertion only by polymerase chain reaction was then designed. The prevalence of strains with 12\u2010bp insertion was examined in 336 strains isolated from O<\/jats:styled-content>kinawa by polymerase chain reaction.<\/jats:p><\/jats:sec>Results<\/jats:title>In case of W<\/jats:styled-content>estern\u2010type cagA<\/jats:italic><\/jats:styled-content>\/vacA<\/jats:italic><\/jats:styled-content> s1m2 strains, the prevalence of 12\u2010bp insertion was significantly higher in strains isolated from O<\/jats:styled-content>kinawa than that from the U<\/jats:styled-content>.S<\/jats:styled-content>. (P<\/jats:italic>\u2009=\u20090.002). Phylogenetic tree showed that strains with 12\u2010bp insertion formed two individual clusters within J<\/jats:styled-content>\u2010W<\/jats:styled-content>estern\u2010type cagA<\/jats:italic><\/jats:styled-content> subtype; one is from O<\/jats:styled-content>kinawa and another is from the U<\/jats:styled-content>.S<\/jats:styled-content>. The designed primer set showed high sensitivity (100%) and specificity (90.8%) in O<\/jats:styled-content>kinawa. The 12\u2010bp insertion was found in 23.7%, 14.3%, 4.2%, and 4.0% of strains with duodenal ulcer (DU<\/jats:styled-content>), gastritis, gastric cancer, and gastric ulcer (GU<\/jats:styled-content>), respectively (P<\/jats:italic>\u2009<\u20090.001 for DU<\/jats:styled-content> vs<\/jats:italic> GU<\/jats:styled-content>) in O<\/jats:styled-content>kinawa.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Although the mechanisms are unknown, the presence of 12\u2010bp insertion was associated with the presence of DU<\/jats:styled-content> and might have a suppressive action on GU<\/jats:styled-content> and gastric cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.12067","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T01:06:06Z","timestamp":1354151166000},"page":"291-296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["H<\/scp>elicobacter pylori<\/i>cagA<\/i><\/scp> 12\u2010bp insertion can be a marker for duodenal ulcer in O<\/scp>kinawa, J<\/scp>apan"],"prefix":"10.1111","volume":"28","author":[{"given":"Yuichi","family":"Matsuo","sequence":"first","affiliation":[{"name":"Departments of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Shiota","sequence":"additional","affiliation":[{"name":"Departments of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"},{"name":"Departments of General Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Matsunari","sequence":"additional","affiliation":[{"name":"Departments of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"},{"name":"Departments of Gastroenterology Oita University Faculty of Medicine Yufu\u2010City Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Departments of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahide","family":"Watada","sequence":"additional","affiliation":[{"name":"Departments of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"},{"name":"Departments of Gastroenterology Oita University Faculty of Medicine Yufu\u2010City Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Thanh","family":"Binh","sequence":"additional","affiliation":[{"name":"Departments of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagisa","family":"Kinjo","sequence":"additional","affiliation":[{"name":"Department of Endoscopy University Hospital University of the Ryukyus Nishihara Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fukunori","family":"Kinjo","sequence":"additional","affiliation":[{"name":"Department of Endoscopy University Hospital University of the Ryukyus Nishihara Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Yamaoka","sequence":"additional","affiliation":[{"name":"Departments of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu\u2010City Oita Japan"},{"name":"Department of Medicine\u2010Gastroenterology Baylor College of Medicine and Michael E. 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