{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:21:02Z","timestamp":1760908862352,"version":"3.37.3"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T00:00:00Z","timestamp":1405382400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81301696"],"award-info":[{"award-number":["81301696"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"crossref","award":["H2012206134"],"award-info":[{"award-number":["H2012206134"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Project of Health Department of Hebei Province"},{"name":"Educational Commission of Hebei Province","award":["2011105"],"award-info":[{"award-number":["2011105"]}]},{"name":"Project of Health Department of Hebei Province","award":["20120269"],"award-info":[{"award-number":["20120269"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2014,7]]},"abstract":"Abstract<\/jats:title>Background and Aim<\/jats:title>The incidence of distal gastric adenocarcinoma has significantly decreased, but gastric cardia adenocarcinoma has been on the rise. Cardia adenocarcinoma might be a specific entity distinct from the carcinoma of the rest stomach. The aim was to explore putative differences in p16INK<\/jats:sup><\/jats:styled-content>4a<\/jats:sup>\u2010retinoblastoma (R<\/jats:styled-content>b) pathway and INK<\/jats:styled-content>4a\/ARF<\/jats:styled-content> methylation between gastric cardia and distal adenocarcinomas.<\/jats:p><\/jats:sec>Methods<\/jats:title>Ninety\u2010six cardia adenocarcinomas and 79 distal samples were analyzed for comparing p16INK<\/jats:sup><\/jats:styled-content>4a<\/jats:sup>\u2010R<\/jats:styled-content>b expressions, INK<\/jats:styled-content>4a\/ARF<\/jats:styled-content> deletion, and methylation using immunohistochemistry, polymerase chain reaction, and methylation\u2010specific polymerase chain reaction.<\/jats:p><\/jats:sec>Results<\/jats:title>The expression of p16INK<\/jats:sup><\/jats:styled-content>4a<\/jats:sup> in cardia adenocarcinoma (43.2%) was significantly lower than in distal cases (75.0%, P<\/jats:styled-content><\/jats:italic>\u2009<\u20090.05). As well, cardia adenocarcinoma showed lower expression of p14ARF<\/jats:sup><\/jats:styled-content> compared with distal cases (34.1% vs<\/jats:italic> 57.5%, P<\/jats:styled-content><\/jats:italic>\u2009<\u20090.05). The incidence of p16INK<\/jats:sup><\/jats:styled-content>4a<\/jats:sup> deletion was 20.5% and 15.0%, while p14ARF<\/jats:sup><\/jats:styled-content> deletion was 18.2% and 10.0% in cardia and distal adenocarcinomas, respectively, showing no significant differences between two entities. However, the incidences of p14ARF<\/jats:sup><\/jats:styled-content> and p16INK<\/jats:sup><\/jats:styled-content>4a<\/jats:sup> methylation in cardia adenocarcinoma were significantly higher than in distal samples (p14ARF<\/jats:sup><\/jats:styled-content>: 61.5% vs<\/jats:italic> 43.6%; p16INK<\/jats:sup><\/jats:styled-content>4a<\/jats:sup>: 73.1% vs<\/jats:italic> 51.3%, P<\/jats:styled-content><\/jats:italic>\u2009<\u20090.05). INK<\/jats:styled-content>4a\/ARF<\/jats:styled-content> methylations were more prevalent in poorly differentiated cardia carcinoma compared with poorly differentiated distal cases.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>There were differences in p16INK<\/jats:sup><\/jats:styled-content>4a<\/jats:sup>\u2010R<\/jats:styled-content>b immunotypes and INK<\/jats:styled-content>4a\/ARF<\/jats:styled-content> methylation between two entities, indicating that cardia adenocarcinoma may be different in cell proliferation, differentiation, and gene biomarkers compared with distal gastric adenocarcinoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.12547","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T12:36:09Z","timestamp":1392208569000},"page":"1418-1426","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Different roles for p16INK<\/sup><\/scp>4a<\/sup>\u2010R<\/scp>b pathway and INK<\/scp>4a\/ARF<\/scp> methylation between adenocarcinomas of gastric cardia and distal stomach"],"prefix":"10.1111","volume":"29","author":[{"given":"Liying","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Pathology Hebei Medical University Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Pathology Hebei Medical University Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology Hebei General Hospital Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinxing","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Pathology Hebei Medical University Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuehong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology The Second Hospital, Hebei Medical University Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingxiao","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Pathology Hebei Medical University Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junling","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Hebei Medical University Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pathology Hebei Medical University Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of 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