{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:51:33Z","timestamp":1791219093718,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,4,27]],"date-time":"2015-04-27T00:00:00Z","timestamp":1430092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology of Japan","doi-asserted-by":"crossref","award":["25462096"],"award-info":[{"award-number":["25462096"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Recent studies show that adipose tissue\u2010derived mesenchymal stem cells have potential clinical applications. However, the mechanism has not been fully elucidated yet. Here, we investigated the effect of basic fibroblast growth factor\u2010treated adipose tissue\u2010derived mesenchymal stem cells infusion on a liver fibrosis rat model and elucidated the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adipose tissue\u2010derived mesenchymal stem cells were infused into carbon tetrachloride\u2010induced hepatic fibrosis rats through caudal vein. Liver functions and pathological changes were assessed. A co\u2010culture model was used to clarify the potential mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Basic fibroblast growth factor treatment markedly improved the proliferation, differentiation, and hepatocyte growth factor expression ability of adipose tissue\u2010derived mesenchymal stem cells. Although adipose tissue\u2010derived mesenchymal stem cells infusion alone slightly ameliorated liver functions and suppressed fibrosis progression, basic fibroblast growth factor\u2010treatment significantly enhanced the therapeutic effect in association with elevated hepatocyte growth factor expression. Moreover, double immunofluorescence staining confirmed that the infused cells located in fibrosis area. Furthermore, co\u2010culture with adipose tissue\u2010derived mesenchymal stem cell led to induction of hepatic stellate cell apoptosis and enhanced hepatocyte proliferation. However, these effects were significantly weakened by knockdown of hepatocyte growth factor. Mechanism investigation revealed that co\u2010culture with adipose tissue\u2010derived mesenchymal stem cells activated c\u2010jun N\u2010terminal kinase\u2010p53 signaling in hepatic stellate cell and promoted apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Basic fibroblast growth factor treatment enhanced the therapeutic effect of adipose tissue\u2010derived mesenchymal stem cells, and secretion of hepatocyte growth factor from adipose tissue\u2010derived mesenchymal stem cells plays a critical role in amelioration of liver injury and regression of fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.12893","type":"journal-article","created":{"date-parts":[[2015,1,16]],"date-time":"2015-01-16T17:00:18Z","timestamp":1421427618000},"page":"1065-1074","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Basic fibroblast growth factor\u2010treated adipose tissue\u2010derived mesenchymal stem cell infusion to ameliorate liver cirrhosis via paracrine hepatocyte growth factor"],"prefix":"10.1111","volume":"30","author":[{"given":"Wei\u2010Ping","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Disaster and Emergency Medicine Faculty of Medicine Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiko","family":"Akahoshi","sequence":"additional","affiliation":[{"name":"Department of Disaster and Emergency Medicine Faculty of Medicine Kyushu University  Fukuoka Japan"},{"name":"Department of Surgery and Science Graduate School of Medical Sciences Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Shu","family":"Piao","sequence":"additional","affiliation":[{"name":"Department of Disaster and Emergency Medicine Faculty of Medicine Kyushu University  Fukuoka 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