{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T02:20:30Z","timestamp":1785205230344,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,4,28]],"date-time":"2016-04-28T00:00:00Z","timestamp":1461801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>The risk of <jats:bold>c<\/jats:bold>holangiocarcinoma (cCC) arising from choledochal cyst (CC\u2010CC) is imminent, if the latter not treated appropriately in time. Epithelial\u2010to\u2010mesenchymal transition (EMT) is considered a critical step for various solid cancers, which is regulated by the microRNA\u2010200 (miR\u2010200) family. The aim of this study was to assess the role of miR\u2010200 family in the pathogenesis of CC\u2010CC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixteen patients with CC\u2010CC were enrolled and 254 patients with conventional cCC served as clinicopathologic controls. Fifty\u2010four cCC were selected to compare the miR\u2010200 family expression and immunohistochemical characteristics. Gain\u2010and loss\u2010of\u2010function studies of miR\u2010200 family were conducted using the cCC cell lines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CC\u2010CC were younger (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), more female\u2010 predominated (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), and rarely associated with lithiasis (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) compared with those of cCC. miR\u2010200 family was down\u2010regulated in CC\u2010CC, while miR\u2010200 family was paradoxically up\u2010regulated in cCC (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). CC\u2010CC exhibited overt overexpression of mesenchymal markers including ZEB1, Twist, Snail, and vimentin as well an aberrant E\u2010cadherin expression in comparison with cCC. <jats:italic>In vitro<\/jats:italic> migration assay showed that cCC cells bearing lower miR\u2010200\u2009s levels exhibited stronger migration ability. Invasive ability of cCC cells was increased after miR\u2010200\u2009s knockdown, accompanied by up\u2010regulation of mesenchymal markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CC\u2010CC was characterized by distinct demographics, precipitating factors, and down\u2010regulation of miR\u2010200 family, compared with those of cCC. The pathogenesis of CC\u2010CC might partly link to the silencing of miR\u2010200 family, acting via ZEB1\u2010directed EMT activation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.13204","type":"journal-article","created":{"date-parts":[[2015,10,19]],"date-time":"2015-10-19T18:48:44Z","timestamp":1445280524000},"page":"1052-1059","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression profile of microRNA\u2010200 family in cholangiocarcinoma arising from choledochal cyst"],"prefix":"10.1111","volume":"31","author":[{"given":"Chia\u2010Hui","family":"Chu","sequence":"first","affiliation":[{"name":"Department of Surgery, Chang Gung Memorial Hospital Chang Gung University Medical College  LinKou Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchi","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Chang Gung Memorial Hospital Chang Gung University Medical College  LinKou Taiwan"},{"name":"Institute of Clinical Medical Sciences, College of Medicine Chang Gung University  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Chang Gung Memorial Hospital Chang Gung University Medical College  LinKou Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Nan","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Surgery, Chang Gung Memorial Hospital Chang Gung University Medical College  LinKou Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tse\u2010Ching","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Chang Gung Memorial Hospital Chang Gung University Medical College  LinKou Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u2010Sen","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Surgery, Chang Gung Memorial Hospital Chang Gung University Medical College  LinKou Taiwan"},{"name":"Institute of Clinical Medical Sciences, College of Medicine Chang Gung University  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.138.3.333"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2007.12.045"},{"issue":"4","key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"S94","DOI":"10.1093\/annonc\/10.suppl_4.S94","article-title":"Choledochal cystic malignancies","volume":"10","author":"Bismuth H","year":"1999","journal-title":"Ann. 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