{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T09:43:24Z","timestamp":1767865404066,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,30]],"date-time":"2016-05-30T00:00:00Z","timestamp":1464566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grants-in-Aid for Scientific Research KAKENHI","award":["23590730 (YD)"],"award-info":[{"award-number":["23590730 (YD)"]}]},{"name":"Grants-in-Aid for Scientific Research KAKENHI","award":["25893280 (SW)"],"award-info":[{"award-number":["25893280 (SW)"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim:<\/jats:title><jats:p>Inflammatory bowel disease is associated with chronic abdominal pain. Transient receptor potential ankyrin 1 (TRPA1) is a well\u2010known pain sensor expressed in primary sensory neurons. Recent studies indicate that reactive oxygen species such as hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) may activate TRPA1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Colonic inflammation was induced by intra\u2010colonic administration of trinitrobenzene sulfate (TNBS) in adult male Sprague\u2013Dawley rats. Visceromotor response (VMR) to colorectal distention (CRD) was recorded to evaluate the visceral hyperalgesia. Rats were sacrificed 1\u2009day after treatment with saline or TNBS; colonic tissues from the inflamed region were removed and then processed to assess the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> content. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> scavenger N\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine or a TRPA1 antagonist, HC\u2010030031, was intravenously administrated to the TNBS\u2010treated rats or saline\u2010treated rats. In a parallel experiment, intra\u2010colonic H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced visceral hyperalgesia in na\u00efve rats and the effect of intravenous HC\u2010030031 were measured based on the VMR to CRD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Trinitrobenzene sulfate treatment resulted in significant increase in VMR to CRD at day 1. The H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> content in the inflamed region of the colon in TNBS\u2010treated rats was significantly higher than that of saline\u2010treated rats. N\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine or HC\u2010030031 significantly suppressed the enhanced VMR in TNBS\u2010treated rats while saline\u2010treated rats remained unaffected. Moreover, blockade of TRPA1 activation by HC\u2010030031 significantly reversed the exogenous H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced visceral hyperalgesia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>These results suggest that H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> content of the colonic tissue is increased in the early stage of TNBS\u2010induced colitis. The increased H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> content may contribute to the visceral hyperalgesia by activating TRPA1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.13226","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T07:00:52Z","timestamp":1447743652000},"page":"1147-1153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Elevated H<sub>2<\/sub>O<sub>2<\/sub> levels in trinitrobenzene sulfate\u2010induced colitis rats contributes to visceral hyperalgesia through interaction with the transient receptor potential ankyrin 1 cation channel"],"prefix":"10.1111","volume":"31","author":[{"given":"Yoko","family":"Kogure","sequence":"first","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy Hyogo University of Health Sciences  Kobe Hyogo 6508530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenglan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy Hyogo University of Health Sciences  Kobe Hyogo 6508530 Japan"},{"name":"Traditional Medicine Research Center, Chinese Medicine Confucius Institute Hyogo College of Medicine  Kobe Hyogo 6508530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koh\u2010ichi","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy Hyogo University of Health Sciences  Kobe Hyogo 6508530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongbiao","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy Hyogo University of Health Sciences  Kobe Hyogo 6508530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy Hyogo University of Health Sciences  Kobe Hyogo 6508530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyoshi","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy Hyogo University of Health Sciences  Kobe Hyogo 6508530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Traditional Medicine Research Center, Chinese Medicine Confucius Institute Hyogo College of Medicine  Kobe Hyogo 6508530 Japan"},{"name":"Department of Anatomy and Neuroscience Hyogo College of Medicine  Nishinomiya Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy Hyogo University of Health Sciences  Kobe Hyogo 6508530 Japan"},{"name":"Traditional Medicine Research Center, Chinese Medicine Confucius Institute Hyogo College of Medicine  Kobe Hyogo 6508530 Japan"},{"name":"Department of Anatomy and Neuroscience Hyogo College of Medicine  Nishinomiya Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.10.001"},{"key":"e_1_2_6_3_1","first-page":"592","article-title":"Managing pain in inflammatory bowel disease","volume":"7","author":"Docherty MJ","year":"2011","journal-title":"Gastroenterol. Hepatol."},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00158-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(04)00150-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02282"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505356102"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705923104"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5369-07.2008"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.153585"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108164"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00221.2009"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2008.06.004"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5149"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24683"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.153585"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-012-1207-9"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.114.217570"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2008.05.093"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2006.02.029"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.05.034"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.179523"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.04.049"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00356.2012"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2992-10.2011"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.002"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.10.014"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI35957"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.107.040832"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90036-K"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)91112-H"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/14734220601142878"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-006-3194-z"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-008-0438-0"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365513.2012.734859"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2008.06093.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.194449"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04503-7"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00351.2007"}],"container-title":["Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjgh.13226","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjgh.13226","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.13226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T10:42:36Z","timestamp":1696156956000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jgh.13226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,30]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/jgh.13226"],"URL":"https:\/\/doi.org\/10.1111\/jgh.13226","archive":["Portico"],"relation":{},"ISSN":["0815-9319","1440-1746"],"issn-type":[{"value":"0815-9319","type":"print"},{"value":"1440-1746","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,30]]},"assertion":[{"value":"2015-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}