{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:09:25Z","timestamp":1787170165119,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aim<\/jats:title>\n                    <jats:p>Human leukocyte antigen (HLA) typing is an important step in the diagnostic algorithm for celiac disease (CD) and is also used for screening purposes. Collection of blood is invasive and accompanied with emotional impact especially in children. Genetic technological progress now enables HLA typing from buccal cell samples. This study evaluated the reliability and feasibility of HLA typing for CD\u2010associated HLA polymorphisms using buccal swabs as routine test in high\u2010risk individuals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood and buccal swabs of 77 children and adolescents with high risk for CD were prospectively collected in this cohort study. Buccal swab collection was performed either by the investigator at the outpatient clinic or by the patient or its parents at home. To evaluate the possibility of self\u2010administration, three families performed the test at home. DNA was extracted using an adapted QIAamp method. Quantity, quality, and purity of DNA were recorded. HLA\u2010DRB1, HLA\u2010DQA1, and HLA\u2010DQB1 typing was examined on buccal cell\u2010derived and blood\u2010derived DNA at low and, if necessary, high resolution level, using sequence\u2010specific oligonucleotide and sequence\u2010based typing, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>DNA isolation using buccal swabs yielded a good quality and sufficient quantity of DNA to perform HLA\u2010DQ typing in all individuals. HLA typing results on buccal cell\u2010derived DNA were identical to typing on blood\u2010derived DNA, also for the self\u2010administered samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Introduction of the buccal swab test for HLA typing of CD risk in routine diagnostics can omit the current venipuncture and enables self\u2010administration at home. Therefore, the buccal swab test is beneficial for individuals with a clinical suspicion for CD, as well as for screening purposes in high\u2010risk populations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jgh.13331","type":"journal-article","created":{"date-parts":[[2016,3,6]],"date-time":"2016-03-06T02:52:19Z","timestamp":1457232739000},"page":"1711-1716","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Human leukocyte antigen typing using buccal swabs as accurate and non\u2010invasive substitute for venipuncture in children at risk for celiac disease"],"prefix":"10.1111","volume":"31","author":[{"given":"Marlou P M","family":"Adriaanse","sequence":"first","affiliation":[{"name":"Department of Pediatrics &amp; Nutrition and Toxicology Research Institute Maastricht (NUTRIM) Maastricht University Medical Center  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita C E","family":"Vreugdenhil","sequence":"additional","affiliation":[{"name":"Department of Pediatrics &amp; Nutrition and Toxicology Research Institute Maastricht (NUTRIM) Maastricht University Medical Center  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Vastmans","sequence":"additional","affiliation":[{"name":"Department of Transplantation Immunology and Tissue Typing Maastricht University Medical Centre  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisette","family":"Groeneveld","sequence":"additional","affiliation":[{"name":"Department of Transplantation Immunology and Tissue Typing Maastricht University Medical Centre  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Molenbroeck","sequence":"additional","affiliation":[{"name":"Department of Transplantation Immunology and Tissue Typing Maastricht University Medical Centre  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina A","family":"Schott","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Atrium Medical Center Parkstad  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina E M","family":"Voorter","sequence":"additional","affiliation":[{"name":"Department of Transplantation Immunology and Tissue Typing Maastricht University Medical Centre  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel G J","family":"Tilanus","sequence":"additional","affiliation":[{"name":"Department of Transplantation Immunology and Tissue Typing Maastricht University Medical Centre  Heerlen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071600"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00321"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1780"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31821a23d0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-5448.2002.30205.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2011.07.003"},{"key":"e_1_2_7_8_1","first-page":"1005","article-title":"Determinants of DNA yield and quality from buccal cell samples collected with mouthwash","volume":"10","author":"Feigelson HS","year":"2001","journal-title":"Cancer Epidemiol. 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