{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T18:55:59Z","timestamp":1766516159396},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,4]],"date-time":"2017-01-04T00:00:00Z","timestamp":1483488000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p><jats:italic>KRAS<\/jats:italic> mutation analysis may provide ancillary diagnostic yield in an endoscopic ultrasound (EUS)\u2010guided histopathological evaluation. We evaluated the clinical impact of <jats:italic>KRAS<\/jats:italic> mutation analysis with cytological and histological evaluations in EUS\u2010guided tissue sampling, using a core biopsy needle for solid liver masses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>EUS\u2010guided fine needle biopsy (EUS\u2010FNB) using a core biopsy needle was performed in patients with primary or metastatic liver masses that were suspected malignancies. <jats:italic>KRAS<\/jats:italic> mutations were analyzed in all specimens obtained. The peptide nucleic acid (PNA)\u2010mediated polymerase chain reaction (PCR) clamping method was compared with a next\u2010generation sequencing (NGS) method for detecting <jats:italic>KRAS<\/jats:italic> mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EUS\u2010FNB with a core biopsy needle was performed in 28 patients with solid liver masses located in the liver's left lobe. The diagnostic accuracies for malignancy of on\u2010site cytology, cytology using Papanicolaou staining, and histology including immunohistochemical stains were 82.1%, 85.7%, and 89.3%, respectively. <jats:italic>KRAS<\/jats:italic> gene mutations were observed in 14.3% of patients by the PNA\u2010PCR clamping method. The NGS method detected more <jats:italic>KRAS<\/jats:italic> mutations than the PNA\u2010PCR clamping method: in 25% of patients. Adding the results of <jats:italic>KRAS<\/jats:italic> mutation analysis to the cytohistopathological evaluation, the overall diagnostic accuracy of EUS\u2010guided tissue sampling was 96.4%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>EUS\u2010guided tissue sampling for solid liver masses in the left lobe provided high diagnostic accuracy from cytological and histological evaluations. <jats:italic>KRAS<\/jats:italic> mutation analysis provided additional diagnostic yield in patients with inconclusive cytohistopathological results from EUS\u2010guided tissue sampling. The NGS method detected additional <jats:italic>KRAS<\/jats:italic> mutations in patients with negative PNA\u2010mediated PCR clamping test results.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.13423","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T14:34:41Z","timestamp":1461767681000},"page":"154-162","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>KRAS<\/i> mutation analysis by next\u2010generation sequencing in endoscopic ultrasound\u2010guided sampling for solid liver masses"],"prefix":"10.1111","volume":"32","author":[{"given":"Hyun Jong","family":"Choi","sequence":"first","affiliation":[{"name":"Digestive Disease Center and Research Institute, Department of Internal Medicine SoonChunHyang University School of Medicine  Bucheon and Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong 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