{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T13:46:18Z","timestamp":1789739178962,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T00:00:00Z","timestamp":1503878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601668"],"award-info":[{"award-number":["81601668"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>A considerable number of early\u2010stage colorectal cancer (CRC) patients may develop cancer relapse or metastasis after curative surgery. Isolated tumor cells (ITC) and micrometastasis in lymph nodes (LNMM), which are undetectable by conventional pathological examination, may be one primary reason. Detection of ITC\/LNMM is time\u2010consuming and cost\u2010ineffective; we aimed to find biomarkers in primary CRC tissues to help predicting ITC\/LNMM status.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled 137 node\u2010negative patients with early\u2010stage CRC in this study. Existence of ITC\/LNMM was identified by immunohistological staining with cytokeratin 20 in resected lymph nodes. Expression of transducin (\u03b2)\u2010like 1 X\u2010linked receptor 1 (TBL1XR1) in primary CRC tissues was also investigated. Chi\u2010squared test was performed to reveal the correlations between ITC\/LNMM and clinicopathological characteristics. Univariate and multivariate analyses were used to determine independent prognostic factors. Knockdown experiment together with proliferation and invasion assays were carried out to explore molecular mechanisms between TBL1XR1 and ITC\/LNMM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>About 29.2% (40\/137) patients were identified as ITC\/LNMM positive, and most of them (32\/40 cases, 80%) showed high TBL1XR1 expression in primary CRC tissues. Both ITC\/LNMM and TBL1XR1 expression were independent prognostic factors for disease relapse or metastasis. <jats:italic>In vitro<\/jats:italic> experiments demonstrated that TBL1XR1 can regulate the expression of vascular endothelial growth factor C and epithelial\u2010mesenchymal transition proteins, thus mediate the process of lymph node metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Identification of ITC\/LNMM is significant in evaluating clinical outcome and guiding adjuvant chemotherapy for early\u2010stage CRC patients. TBL1XR1 overexpression in CRC tissues can serve as an efficient biomarker to predict the status of ITC\/LNMM.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.13749","type":"journal-article","created":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T10:05:56Z","timestamp":1485511556000},"page":"1570-1580","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["TBL1XR1 predicts isolated tumor cells and micrometastasis in patients with TNM stage I\/II colorectal cancer"],"prefix":"10.1111","volume":"32","author":[{"given":"Hongda","family":"Liu","sequence":"first","affiliation":[{"name":"Department of General Surgery Qilu Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaochen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangshuai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Li","sequence":"additional","affiliation":[{"name":"302 Military Hospital of China  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Urological Organ Transplantation The Second Xiangya Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Medicine Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weichen","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Qianfoshan Hospital, Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Qianfoshan Hospital, Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanglei","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayed","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61649-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21208"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.8.583"},{"key":"e_1_2_6_5_1","volume-title":"UICC: TNM Classification of Malignant Tumours","author":"Sobin LHGM","year":"2009"},{"key":"e_1_2_6_6_1","first-page":"602","article-title":"More extensive nodal dissection improves survival for stages I to III of colon cancer: a population\u2010based study","volume":"244","author":"Chen SL","year":"2006","journal-title":"Ann. 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