{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T08:20:44Z","timestamp":1765268444935},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,1,26]],"date-time":"2018-01-26T00:00:00Z","timestamp":1516924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Progesterone receptor, inflammation, neurotransmitter expression, and fibrosis are involved in slow\u2010transit constipation. The aim of the present study was to examine whether patients with slow\u2010transit constipation have an overexpression of progesterone receptor and serotonin, which may impair the fibrosis of muscularis propria in colorectal wall.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>High\u2010resolution colon manometry was used to record the colorectal peristaltic contractions of the proximal ascending and sigmoid colon in patients. Protein samples prepared from frozen sigmoid colon tissue and the proximal margin of the ascending colon of four female patients were compared using isobaric tags for relative and absolute quantification labeling technique coupled to 2D liquid chromatography\u2013tandem mass spectrometry analysis. Immunohistochemical staining of progesterone receptor, serotonin, and fibronectin was performed in paraffin\u2010embedded sigmoid colon tissues and the proximal margin of the ascending colon or ileum from 43 patients with slow\u2010transit constipation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among these differentially regulated proteins based on isobaric tags for relative and absolute quantification and liquid chromatography\u2013tandem mass spectrometry analysis, 56 proteins involved in the response to progesterone, inflammation, matrix remodeling, fibrosis, and muscle metabolism. Immunohistochemical staining confirmed that there was significantly higher expression of progesterone receptor (<jats:italic>t<\/jats:italic>\u00a0=\u00a019.19, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.000) and serotonin (<jats:italic>t<\/jats:italic>\u00a0=\u00a013.52, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.004) in sigmoid colon than in the proximal margin of the ascending colon and ileum. Progesterone receptor and fibronectin expression in the outer layer of muscularis propria were higher than in the middle layer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrate that progesterone receptor, along with inflammation and fibrosis, may take part in slow\u2010transit constipation development.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.13873","type":"journal-article","created":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T02:12:03Z","timestamp":1499825523000},"page":"385-392","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Isobaric tags for relative and absolute quantification\u2010based proteomic analysis that reveals the roles of progesterone receptor, inflammation, and fibrosis for slow\u2010transit constipation"],"prefix":"10.1111","volume":"33","author":[{"given":"Yuwei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xipeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiwu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Union Medical Center  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.27.1.41"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8553(05)70168-0"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-009-2363-5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1754.2001.00692.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH797>3.0.CO;2-C"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005665218647"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2010.03.006"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.2002.02213.x"},{"key":"e_1_2_5_10_1","first-page":"71","article-title":"Colectomy for slow transit constipation","volume":"69","author":"Walsh PV","year":"1987","journal-title":"Ann. 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