{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:34:32Z","timestamp":1777451672168,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T00:00:00Z","timestamp":1526342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000589","name":"Chief Scientist Office","doi-asserted-by":"publisher","award":["0390093"],"award-info":[{"award-number":["0390093"]}],"id":[{"id":"10.13039\/501100000589","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Non\u2010alcoholic fatty liver disease (NAFLD) is associated with all features of the metabolic syndrome. Deposition of excess triglycerides in liver cells, a hallmark of NAFLD, is associated with loss of insulin sensitivity. Ostreolysin (Oly) is a 15\u2010kDa fungal protein known to interact with cholesterol\u2010enriched raft\u2010like membrane domains. We aim to test whether a recombinant version of Oly (rOly) can induce functional changes <jats:italic>in vitro<\/jats:italic> in adipocytes or <jats:italic>in vivo<\/jats:italic> in mice fed a high\u2010fat diet (HFD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>White preadipocyte 3T3\u2010L1 cells or mouse primary adipocytes treated with rOly. Male C57BL\/6 mice were fed a control or HFD and treated with saline or with rOly (1\u00a0mg\/kg BW) every other day for 4\u00a0weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>White preadipocyte 3T3\u2010L1 cells or mouse primary adipocytes treated with rOly acquire a browning phenotype through activation of 5\u2032 adenosine monophosphate\u2010activated protein kinase and downregulation of <jats:styled-content style=\"fixed-case\">tumor necrosis factor<\/jats:styled-content> \u03b1\u2010mediated activation of I\u03baB kinase \u03b5 and TANK\u2010binding kinase 1. HFD\u2010fed mice treated with rOly showed a 10% reduction in BW and improved glucose tolerance, which paralleled improved expression of liver and adipose functionality, metabolism, and inflammation status, mimicking the <jats:italic>in vitro<\/jats:italic> findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides first evidence of rOly's prevention of HFD\u2010induced NAFLD by stimulating liver and adipose muscle tissue functionality and oxidative potential, improving glucose tolerance, and ameliorating the metabolic profile of diet\u2010induced obese mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.14259","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T02:28:18Z","timestamp":1523932098000},"page":"1990-2000","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Ostreolysin induces browning of adipocytes and ameliorates hepatic steatosis"],"prefix":"10.1111","volume":"33","author":[{"given":"Lili","family":"Nimri","sequence":"first","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Staikin","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irena","family":"Peri","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Einav","family":"Yehuda\u2010Shnaidman","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition, The Robert H. 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