{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:07:09Z","timestamp":1788433629237,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,31]],"date-time":"2019-03-31T00:00:00Z","timestamp":1553990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>The pathological features of non\u2010alcoholic steatohepatitis (NASH) have not been determined, so fundamental treatment has not been established. Adipose\u2010tissue\u2010derived stromal\/stem cells (ADSCs) are beneficial for repair\/regenerative therapy of impaired organs because of their immuno\u2010modulatory capability. In this study, we assessed how liver damage progresses during the early development phase of the murine NASH model and investigated whether ADSCs are preventatively efficacious against the fibrosis progression of NASH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C57BL\/6J mice were fed with atherogenic high fat or high\u2010fat diet 60 developing into NASH or simple steatosis. Their hepatic inflammatory cells (HICs) were analyzed by cDNA microarray. NASH mice were treated with ADSCs injected into spleen when hepatic inflammation was initially observed, and liver samples were analyzed. The effect of ADSCs on the mice hepatic stellate cell (HSC) line stimulated by recombinant IL\u201017 and HICs from NASH mice was analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The gene expression features of HICs implicated as humoral cytokine mediators of lymphoid cells during NASH development, compared with a simple steatosis model. One of the featured cytokines was IL\u201017. The development of hepatic fibrosis was alleviated when NASH mice were treated with ADSCs as well as treated with anti\u2010IL\u201017 antibody, and the frequency of IL\u201017\u2010secreting HICs decreased. NASH\u2010HICs enhanced proliferation of HSCs, in which proliferation was sensitive to IL\u201017 stimulation. The stimulatory effect of NASH\u2010HICs on the activation of HSCs was attenuated by co\u2010culture with ADSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ADSCs treatment prevented progression of NASH fibrosis by suppressing IL\u201017\u2010mediated inflammation, which was associated with HSCs activation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.14647","type":"journal-article","created":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T04:44:18Z","timestamp":1551674658000},"page":"1432-1440","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Adipose tissue\u2010derived stem cells prevent fibrosis in murine steatohepatitis by suppressing IL\u201017\u2010mediated inflammation"],"prefix":"10.1111","volume":"34","author":[{"given":"Masatoshi","family":"Yamato","sequence":"first","affiliation":[{"name":"Department of Disease Control and Homeostasis, College of Medical Pharmaceutical and Health Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatsune","family":"Mochida","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Kawaguchi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Takamura","sequence":"additional","affiliation":[{"name":"Department of Cardiology Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichiro","family":"Usui","sequence":"additional","affiliation":[{"name":"Department of Cardiology Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Seki","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eishiro","family":"Mizukoshi","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of General Medicine Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousuke","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2550-2317","authenticated-orcid":false,"given":"Alessandro","family":"Nasti","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho Thuy Bich","family":"Tuyen","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Komura","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Nephrology Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Honda","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Kanazawa University Hospital  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Disease Control and Homeostasis, College of Medical Pharmaceutical and Health Sciences Kanazawa University  Kanazawa Japan"},{"name":"Department of Gastroenterology Kanazawa University Hospital  Kanazawa Japan"},{"name":"Department of System Biology, Graduate School of Advanced Preventive Medical Sciences Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,31]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130246"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60121-5"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000444551"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/esmoopen-2016-000042"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.10.013"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28677"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2011.50"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-017-0806-8"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1999.01377.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0197"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2008.05496.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21704"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343531"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-02-0105"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26470"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-012-0614-6"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201646835"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.31.391"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.10.002"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26746"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2012.05.049"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.024935"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00414.2010"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2017.31"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2016.16.3.147"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-013-9468-6"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.02.026"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-15022-3"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2009.00564.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2011.10.012"}],"container-title":["Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjgh.14647","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.14647","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jgh.14647","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111%2Fjgh.14647","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.14647","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T06:23:47Z","timestamp":1694327027000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jgh.14647"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,31]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1111\/jgh.14647"],"URL":"https:\/\/doi.org\/10.1111\/jgh.14647","archive":["Portico"],"relation":{},"ISSN":["0815-9319","1440-1746"],"issn-type":[{"value":"0815-9319","type":"print"},{"value":"1440-1746","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,31]]},"assertion":[{"value":"2018-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}