{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:05:19Z","timestamp":1787047519305,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T00:00:00Z","timestamp":1563235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["104\u20102314\u2010B\u2010075B\u2010001\u2010MY2"],"award-info":[{"award-number":["104\u20102314\u2010B\u2010075B\u2010001\u2010MY2"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Anti\u2010<jats:styled-content style=\"fixed-case\"><jats:italic>Helicobacter pylori<\/jats:italic><\/jats:styled-content> therapy may lead to the growth of pathogenic or antibiotic\u2010resistant bacteria in the gut. The study aimed to investigate the short\u2010term and long\u2010term impacts of <jats:styled-content style=\"fixed-case\"><jats:italic>H.\u00a0pylori<\/jats:italic><\/jats:styled-content> eradication with reverse hybrid therapy on the components and macrolide resistance of the gut microbiota.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Helicobacter pylori<\/jats:italic><\/jats:styled-content>\u2010related gastritis patients were administered a 14\u2010day reverse hybrid therapy. Fecal samples were collected before treatment and at the end of week 2, week 8, and week 48. The V3\u2013V4 region of the bacterial <jats:italic>16S rRNA<\/jats:italic> gene in fecal specimens was amplified by polymerase chain reaction and sequenced on Illumina MiSeq platform. Additionally, amplification of <jats:italic>erm<\/jats:italic>(B) gene (encoding erythromycin resistance methylase) was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Reverse hybrid therapy resulted in decreased relative abundances of Firmicutes (from 62.0% to 30.7%; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and Actinobacteria (from 3.4% to 0.6%; 0.032) at the end of therapy. In contrast, the relative abundance of Proteobacteria increased from 10.2% to 49.1% (0.002). These microbiota alterations did not persist but returned to the initial levels at week 8 and week 48. The amount of <jats:italic>erm<\/jats:italic>(B) gene in fecal specimens was comparable with the pretreatment level at week 2 but increased at week 8 (0.025) and then returned to the pretreatment level by week 48.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Helicobacter pylori<\/jats:italic><\/jats:styled-content> eradication with reverse hybrid therapy can lead to short\u2010term gut dysbiosis. The amount of <jats:italic>erm<\/jats:italic>(B) gene in the stool increased transiently after treatment and returned to the pretreatment level at 1\u2010year post\u2010treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.14736","type":"journal-article","created":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T09:38:40Z","timestamp":1558517920000},"page":"1968-1976","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Short\u2010term and long\u2010term impacts of <scp><i>Helicobacter pylori<\/i><\/scp> eradication with reverse hybrid therapy on the gut microbiota"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3905-4674","authenticated-orcid":false,"given":"Ping\u2010I","family":"Hsu","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine Kaohsiung Veterans General Hospital and National Yang\u2010Ming University  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