{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:10:11Z","timestamp":1789585811145,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatic fibrosis is the final endpoint for most chronic liver diseases and remains a significant public health problem worldwide. Icariin, a naturally occurring flavonol glucoside, has been reported to exhibit protective effects on liver injury and alleviate liver fibrosis. However, the underlying detail molecular mechanism is not fully revealed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mouse primary hepatic stellate cells (HSCs) and carbon tetrachloride (CCL<jats:sub>4<\/jats:sub>)\u2010induced liver fibrosis model in mice were used as <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> models in this study. The expression levels of miR\u2010875\u20105p were detected by quantitative reverse transcription\u2010PCR. The validation of the direct target of miR\u2010875\u20105p was through dual\u2010luciferase reporter assay and western blotting assay. The cell proliferation and cell mobility were determined using MTT assay and Transwell migration assay, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that icariin inhibited epithelial\u2013mesenchymal transition and collagen protein section of HSCs. Icariin exerted hepatoprotective effects on mice model of CCL<jats:sub>4<\/jats:sub>\u2010induced liver fibrosis. Our further results revealed that miR\u2010875\u20105p was downregulated in human cirrhosis tissues and activated murine HSCs. Icariin induced miR\u2010875\u20105p upregulation and subsequently decreased glioma\u2010associated oncogene homolog 1 (GLI1) expression through direct binding to the three prime untranslated region of GLI1 mRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study highlighted the potential therapeutic application of icariin for liver fibrosis management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.14875","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T21:51:17Z","timestamp":1571262677000},"page":"482-491","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Icariin\u2010induced miR\u2010875\u20105p attenuates epithelial\u2010mesenchymal transition by targeting hedgehog signaling in liver fibrosis"],"prefix":"10.1111","volume":"35","author":[{"given":"Lei","family":"Ye","sequence":"first","affiliation":[{"name":"First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Yu","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0224-0417","authenticated-orcid":false,"given":"Yanping","family":"Zhao","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24282"},{"key":"e_1_2_7_3_1","first-page":"737","article-title":"Reversibility of liver fibrosis","volume":"9","author":"Brenner DA","year":"2013","journal-title":"Gastroenterol. 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