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This study aimed to investigate the gastric cardiac microbiome and identify inflammation\u2010related microorganisms for gastric cardiac inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed 16S rRNA sequencing on a total of 11 healthy individuals and 89 individuals with different degree of gastric cardiac inflammation. Immunohistochemistry was used for verifying candidate bacteria. Phylogenetic reconstruction of unobserved states (<jats:sc>picrust<\/jats:sc>) was used for predicting the pathways involved by cardiac microflora.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The resident phyla in normal were <jats:italic>Proteobacteria<\/jats:italic>, <jats:italic>Firmicutes<\/jats:italic>, <jats:italic>Bacteroides<\/jats:italic>, and <jats:italic>Actinobacteria<\/jats:italic>, and the dominant genus in normal were <jats:italic>Halomonas<\/jats:italic>, <jats:italic>shewanella<\/jats:italic>, and <jats:italic>Comamonas<\/jats:italic>. In the progression of gastric cardiac inflammation, the diversity of cardiac microflora did not change (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05). However, the composition structure of cardiac microflora varied between healthy and inflamed tissues (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Meanwhile, there were 64 species parallel increased with inflammation degree, especially <jats:italic>Helicobacter pylori, Lactobacillus<\/jats:italic> spp. Additionally, inflammation\u2010related species were detected (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), including <jats:italic>H. pylori<\/jats:italic>, <jats:italic>Acinetobacter ursingii<\/jats:italic>, and <jats:italic>Streptococcus agalactiae<\/jats:italic>. Higher <jats:italic>H.\u00a0pylori<\/jats:italic> colonization was positively related to the progression of cardiac inflammation (<jats:italic>\u03b3<\/jats:italic> coefficient\u00a0=\u00a00.678, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), and it also influenced the cardiac microbial community structure. Cardiac microflora also participated in DNA repair pathways and is affected by the relative abundance of <jats:italic>H.\u00a0pylori<\/jats:italic> (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cardiac microflora dysbiosis, especially the increasing of the relevant abundance of <jats:italic>H. pylori<\/jats:italic>, promotes the progression of cardiac inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.14946","type":"journal-article","created":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T22:24:38Z","timestamp":1575066278000},"page":"1032-1041","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Microbiological evidences for gastric cardiac microflora dysbiosis inducing the progression of 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