{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:26:07Z","timestamp":1780629967331,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T00:00:00Z","timestamp":1601424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>The inflammasomes promote pro\u2010caspase\u20101 cleavage, leading to processing of pro\u2010interleukin (IL)\u20101\u03b2 into its mature form. We investigated the role of the IL\u20101\u03b2 and nucleotide binding oligomerization domain\u2010like receptor protein 3 (NLRP3) inflammasome in gastric injury in mice receiving water\u2010immersion restraint stress (WIRS), focusing on the cyclooxygenase (COX)\u20102\/prostaglandin (PG) E<jats:sub>2<\/jats:sub> axis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To induce gastric injury, the mice were placed in a restraint cage and immersed in the water bath to the level of the xiphoid process. Protein levels of mature caspase\u20101 and IL\u20101\u03b2 were assessed by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Water\u2010immersion restraint stress induced gastric injury with increase in IL\u20101\u03b2 expression by activation of NLRP3 inflammasome. Exogenous IL\u20101\u03b2 attenuated the injury, whereas anti\u2010IL\u20101\u03b2 neutralizing antibody and IL\u20101\u03b2 receptor antibody aggravated it. NLRP3<jats:sup>\u2212\/\u2212<\/jats:sup> and caspase\u20101<jats:sup>\u2212\/\u2212<\/jats:sup> mice enhanced the injury with reducing of mature IL\u20101\u03b2, and this aggravation was reduced by exogenous IL\u20101\u03b2 supplementation. Toll\u2010like receptor 4<jats:sup>\u2212\/\u2212<\/jats:sup> mice were hyporesponsive to WIRS in terms of mature IL\u20101\u03b2 production. Rabeprazole attenuated the injury with preventing inflammasome activation. WIRS injured the stomach with promotion of COX\u20102 mRNA and PGE<jats:sub>2<\/jats:sub> production, and exogenous IL\u20101\u03b2 enhanced these molecules, while IL\u20101\u03b2 immunoneutralization exerted opposite effect. PGE<jats:sub>2<\/jats:sub> supplementation abolished the hypersensitivity in NLRP3<jats:sup>\u2212\/\u2212<\/jats:sup> and caspase\u20101<jats:sup>\u2212\/\u2212<\/jats:sup> mice through negative regulation of inflammatory cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that NLRP3 inflammasome\u2010derived IL\u20101\u03b2 plays a protective role in stress\u2010induced gastric injury via activation of the COX\u20102\/PGE<jats:sub>2<\/jats:sub> axis. Toll\u2010like receptor 4 signaling and gastric acid may be involved in NLRP3 inflammasome activation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15257","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T15:43:24Z","timestamp":1601048604000},"page":"740-750","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Role of nucleotide binding oligomerization domain\u2010like receptor protein 3 inflammasome in stress\u2010induced gastric injury"],"prefix":"10.1111","volume":"36","author":[{"given":"Akira","family":"Higashimori","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8567-0585","authenticated-orcid":false,"given":"Toshio","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Nadatani","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akinobu","family":"Nakata","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-6415","authenticated-orcid":false,"given":"Koji","family":"Otani","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8808-5672","authenticated-orcid":false,"given":"Shuhei","family":"Hosomi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumio","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Kamata","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Taira","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuaki","family":"Nagami","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9305-1637","authenticated-orcid":false,"given":"Tetsuya","family":"Tanigawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000201464"},{"key":"e_1_2_6_3_1","first-page":"423","article-title":"The role of reactive oxygen species and capsaicin\u2010sensitive sensory nerves in the pathomechanisms of gastric ulcers induced by stress","volume":"54","author":"Kwiecien S","year":"2003","journal-title":"J. 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