{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T23:39:27Z","timestamp":1785800367953,"version":"3.56.0"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Studies on the resolution of liver fibrosis are becoming more important in this era of etiologic eradication. In contrast to the extensive research on the recovery of liver fibrosis induced by hepatotoxic injuries, regression of cholestatic liver fibrosis has been insufficiently examined owing to the limited availability of animal models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined our novel recanalization mice model of biliary obstruction, involving anastomosis between the gallbladder and jejunum (G\u2013J anastomosis) by invagination. Transgenic mice expressing green fluorescent protein (GFP) under the collagen 1(\u03b1)1 promoter underwent G\u2013J anastomosis 14\u00a0days after bile duct ligation (BDL) and were sacrificed 14\u00a0days later.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transaminase and total bilirubin levels decreased to almost normal values on day 14 after G\u2013J anastomosis. G\u2013J anastomosis resulted in dramatic reversal of liver fibrosis induced by BDL. Activated portal fibroblasts (PFs) double\u2010positive for GFP and Thy\u20101 on immunofluorescence in the liver of BDL\u2010injured mice became less noticeable following G\u2013J anastomosis. Messenger RNA expression of markers for activated PFs in the liver was downregulated after anastomosis. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) were induced by BDL. After anastomosis, expressions of MMP\u20103, 8 as well as hepatocyte growth factor were further upregulated, whereas those of TIMP\u20101 and TIMP\u20103 were markedly downregulated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our established G\u2013J anastomosis model is associated with fibrosis resolution and reduced PF activation through reopening of bile duct obstruction and will be valuable for studying the recovery process of cholestatic liver fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15406","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T23:39:39Z","timestamp":1610753979000},"page":"2493-2500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Novel mouse model for cholestasis\u2010induced liver fibrosis resolution by 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