{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:35:21Z","timestamp":1783427721928,"version":"3.54.6"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,2,28]],"date-time":"2021-02-28T00:00:00Z","timestamp":1614470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Non\u2010alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to non\u2010alcoholic steatohepatitis, which is characterized by hepatic inflammation that can progress to fibrosis, cirrhosis, and hepatocellular carcinoma. Visfatin, an adipocytokine, was reported to induce pro\u2010inflammatory cytokines and can be associated with liver fibrosis. We investigated the role of visfatin on hepatic inflammation and fibrosis in a methionine\u2010choline\u2010deficient (MCD)\u2010diet\u2010induced steatohepatitis mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eight\u2010week\u2010old male C57BL\/6 J mice were randomly assigned into one of three groups: (1) saline\u2010injected control diet group; (2) saline\u2010injected MCD diet group; and (3) visfatin\u2010injected MCD diet group (<jats:italic>n<\/jats:italic>\u00a0=\u00a08 per group). Mice were administered intravenous saline or 10\u00a0\u03bcg\/kg of recombinant murine visfatin for 2\u00a0weeks. Histologic assessment of liver and biochemical and molecular measurements of endoplasmic reticulum (ER) stress, reactive oxidative stress (ROS), inflammation, and fibrosis were performed in livers from these animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Visfatin injection aggravated hepatic steatosis and increased plasma alanine aminotransferase and aspartate aminotransferase concentrations. Visfatin increased inflammatory cell infiltration (as indicated by F4\/80, CD68, ly6G, and CD3 mRNA expression) and expression of chemokines in the liver. Visfatin also increased the expression of pro\u2010inflammatory cytokines (IL\u20101\u03b2, TNF\u2010\u03b1, and IL\u20106) and activated fibrosis markers (CTGF, TIMP1, collagen 1\u03b12, collagen 3\u03b12, \u03b1SMA, fibronectin, and vimentin) in liver. Livers of visfatin\u2010injected mice showed upregulation of ER stress and ROS and activation of JNK signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that visfatin aggravates hepatic inflammation together with induction of ER and oxidative stress and exacerbates fibrosis in an MCD\u2010diet\u2010fed mouse model of NAFLD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15465","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T21:35:45Z","timestamp":1613684145000},"page":"2592-2600","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Visfatin exacerbates hepatic inflammation and fibrosis in a methionine\u2010choline\u2010deficient diet mouse model"],"prefix":"10.1111","volume":"36","author":[{"given":"Yu Jung","family":"Heo","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences Graduate School of Ajou University  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010E","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Physiology Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nami","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ja Young","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Jin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yup","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Physiology Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwan Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8958-7164","authenticated-orcid":false,"given":"Hae Jin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Ajou University School of Medicine  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2016.05.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2008.05543.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M800042-JLR200"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047-783X-18-12"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1255355"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2015.06.011"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1997-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.1201"},{"key":"e_1_2_6_10_1","first-page":"28","article-title":"Correlation of visfatin level with non\u2010alcoholic fatty liver in metabolic syndrome","volume":"31","author":"Mousavi Z","year":"2017","journal-title":"Med. 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