{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T21:30:22Z","timestamp":1789075822287,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T00:00:00Z","timestamp":1615248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>To determine the application range of diagnostic kits utilizing anti\u2010<jats:styled-content style=\"fixed-case\"><jats:italic>Helicobacter pylori<\/jats:italic><\/jats:styled-content> antibody, we tested a newly developed latex aggregation turbidity assay (latex) and a conventional enzyme\u2010linked immunosorbent assay (E\u2010plate), both containing Japanese <jats:styled-content style=\"fixed-case\"><jats:italic>H.\u00a0pylori<\/jats:italic><\/jats:styled-content> protein lysates as antigens, using sera from seven Asian countries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum samples (1797) were obtained, and standard <jats:styled-content style=\"fixed-case\"><jats:italic>H.\u00a0pylori<\/jats:italic><\/jats:styled-content> infection status and atrophy status were determined by culture and histology (immunohistochemistry) using gastric biopsy samples from the same individuals. The two tests (enzyme\u2010linked immunosorbent assay and latex) were applied, and receiver operating characteristics analysis was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Area under the curve (AUC) from the receiver operating characteristic of E\u2010plate and latex curves were almost the same and the highest in Vietnam. The latex AUC was slightly lower than the E\u2010plate AUC in other countries, and the difference became statistically significant in Myanmar and then Bangladesh as the lowest. To consider past infection cases, atrophy was additionally evaluated. Most of the AUCs decreased using this atrophy\u2010evaluated status; however, the difference between the two kits was not significant in each country, but the latex AUC was better using all samples. Practical cut\u2010off values were 3.0\u00a0U\/mL in the E\u2010test and 3.5\u00a0U\/mL in the latex test, to avoid missing gastric cancer patients to the greatest extent possible.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The kits were applicable in all countries, but new kits using regional <jats:styled-content style=\"fixed-case\"><jats:italic>H.\u00a0pylori<\/jats:italic><\/jats:styled-content> strains are recommended for Myanmar and Bangladesh. Use of a cut\u2010off value lower than the best cut\u2010off value is essential for screening gastric cancer patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15467","type":"journal-article","created":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T04:09:18Z","timestamp":1613880558000},"page":"2198-2209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Serum <scp><i>Helicobacter pylori<\/i><\/scp> antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2663-9358","authenticated-orcid":false,"given":"Junko","family":"Akada","sequence":"first","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Faculty of Medicine Oita University  Yufu 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Medicine, Faculty of Medicine Oita University  Yufu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phawinee","family":"Subsomwong","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Faculty of Medicine Oita University  Yufu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Langgeng Agung","family":"Waskito","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Faculty of Medicine Oita University  Yufu Japan"},{"name":"Institute of Tropical Disease Airlangga University  Surabaya Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Thanh","family":"Binh","sequence":"additional","affiliation":[{"name":"Department of Endoscopy Cho Ray Hospital  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lam Tung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of 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