{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:25:46Z","timestamp":1776875146587,"version":"3.51.2"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013105","name":"Shanghai Rising-Star Program","doi-asserted-by":"publisher","award":["13QA1400700"],"award-info":[{"award-number":["13QA1400700"]}],"id":[{"id":"10.13039\/501100013105","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270007"],"award-info":[{"award-number":["81270007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670513"],"award-info":[{"award-number":["81670513"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and aim<\/jats:title><jats:p>Activated hepatic stellate cells (HSCs) are the most critical cells responsible for liver fibrosis, and platelet\u2010derived growth factor (PDGF) is the most prominent mitogen for HSCs in fibrogenesis. This study aimed to explore the potential of gadolinium (Gd)\u2010labeled cyclic peptides (pPB) targeting PDGF receptor\u2010\u03b2 (PDGFR\u2010\u03b2) as a magnetic resonance imaging (MRI) radiotracer to identify the progression of liver fibrosis by imaging hepatic PDGFR\u2010\u03b2 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice treated with carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) were used to mimic hepatic fibrosis <jats:italic>in vivo<\/jats:italic>. The binding activity of FITC\u2010labeled pPB to PDGFR\u2010\u03b2 was assessed in cultured human HSCs (HSC\u2010LX2). MRI was performed to visualize hepatic PDGFR\u2010\u03b2 expression in mice with different degrees of liver fibrosis after Gd\u2010labeled pPB was injected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hepatic PDGFR\u2010\u03b2 expression level was correlated with the severity of liver fibrosis, and the majority of cells expressing PDGFR\u2010\u03b2 were found to be activated HSCs in fibrotic livers. Culture\u2010activated human HSCs expressed abundant PDGFR\u2010\u03b2, and FITC\u2010labeled pPB could bind to these cells in a concentration\u2010dependent and time\u2010dependent manner. With Gd\u2010labeled pPB as a tracer, an MRI modality demonstrated that the relative hepatic T1\u2010weighted MRI signal value progressively increased with the severity of hepatic fibrosis and reduced with remission.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hepatic PDGFR\u2010\u03b2 expression reflects the progression of hepatic fibrosis, and MRI using Gd\u2010labeled pPB as a tracer exhibits potential for distinguishing liver fibrosis staging in mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15628","type":"journal-article","created":{"date-parts":[[2021,7,19]],"date-time":"2021-07-19T08:16:14Z","timestamp":1626682574000},"page":"3448-3456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A magnetic resonance imaging modality for non\u2010invasively distinguishing progression of liver fibrosis by visualizing hepatic platelet\u2010derived growth factor receptor\u2010beta 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Smart Drug Delivery, Ministry of Education and PLA, School of Pharmacy Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7722-8327","authenticated-orcid":false,"given":"Sheng\u2010xiang","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Radiology, Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010yao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Zhongshan Hospital Fudan University  Shanghai 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