{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:26:04Z","timestamp":1783571164259,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,13]],"date-time":"2022-02-13T00:00:00Z","timestamp":1644710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF1000603"],"award-info":[{"award-number":["2021YFF1000603"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82000498"],"award-info":[{"award-number":["82000498"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073352"],"award-info":[{"award-number":["82073352"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Colonic stem cells play important roles in both normal epithelial turnover and injury repair. Lgr5<jats:sup>+<\/jats:sup> colonic stem cells are highly susceptible to DSS\u2010induced damage. However, it is still unclear how colonic stem cells regenerate injured epithelium during colitis. Here, we explored the functions of a new population of NFATc1<jats:sup>+<\/jats:sup> colonic stem cells in experimental colitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nfatc1<jats:sup>+<\/jats:sup> colonic stem cells were labeled using <jats:italic>Nfatc1<\/jats:italic><jats:sup><jats:italic>CreERT2<\/jats:italic><\/jats:sup>;<jats:italic>R26<\/jats:italic><jats:sup><jats:italic>mTmG<\/jats:italic><\/jats:sup> reporter mice. Immunostaining assays were used to detect Goblet cells, enteroendocrine cells, and intestinal stem\/progenitor cells. We performed lineage tracing assay to investigate whether Nfatc1<jats:sup>+<\/jats:sup> cells are real colonic stem cells using <jats:italic>Nfatc1<\/jats:italic><jats:sup><jats:italic>CreERT2<\/jats:italic><\/jats:sup>;<jats:italic>R26<\/jats:italic><jats:sup><jats:italic>mTmG<\/jats:italic><\/jats:sup> mice. The contribution of Nfatc1<jats:sup>+<\/jats:sup> stem cells on epithelial regeneration was detected in experimental colitis induced by DSS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nfatc1\u2010reporter marked cells are enriched for +3 to +5 position in colonic crypts, and they are overlapped with Sox9<jats:sup>+<\/jats:sup> cells and Hopx<jats:sup>+<\/jats:sup> cells that have been identified as stem cells in small intestine. However, Nfatc1\u2010reporter marked cells are not overlapped with Lgr5<jats:sup>+<\/jats:sup> colonic stem cells, as well as differentiated goblet cells and enteroendocrine cells. Furthermore, Nfatc1\u2010reporter marked cells are able to give rise to all lineages of the colonic epithelium, and they preferentially contribute to the regeneration of colonic epithelium in DSS\u2010induced experimental colitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Nfatc1<jats:sup>+<\/jats:sup> cells were identified as a novel population of colonic stem cells that are primarily located at +3 to +5 position and contribute to epithelial regeneration during colitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15790","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T03:13:45Z","timestamp":1643685225000},"page":"734-740","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nfatc1<sup>+<\/sup> colonic stem cells contribute to regeneration upon colitis"],"prefix":"10.1111","volume":"37","author":[{"given":"Jianyang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linkuo","family":"Shang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8696-2013","authenticated-orcid":false,"given":"Zhengquan","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratories for Agrobiotechnology, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqiang","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jin","sequence":"additional","affiliation":[{"name":"National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital and Shenzhen Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201899984"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06196"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1013004108"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.165"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.02.042"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1213214"},{"key":"e_1_2_6_8_1","first-page":"1","article-title":"Hallmarks of 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