{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T01:29:57Z","timestamp":1774574997501,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17888278"],"award-info":[{"award-number":["17888278"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15553065"],"award-info":[{"award-number":["15553065"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Chronic enteropathy associated with the solute carrier organic anion transporter family member 2A1 (SLCO2A1), or CEAS, causes anemia and hypoalbuminemia in young people. Dysfunction of the SLCO2A1 transporter protein is thought to involve genetic mutation, but mutant proteins have not been functionally characterized. We examined the prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) transport ability of recombinant SLCO2A1 proteins containing 11 <jats:italic>SLCO2A1<\/jats:italic> mutations found in CEAS patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Wild\u2010type and mutant SLCO2A1 proteins were forcibly expressed in <jats:styled-content style=\"fixed-case\"><jats:italic>Xenopus laevis<\/jats:italic><\/jats:styled-content> oocytes, and measurements of PGE<jats:sub>2<\/jats:sub> uptake and transport capacity were compared. The membrane protein topology and functionality of the eight <jats:italic>SLCO2A1<\/jats:italic> mutations involving single\u2010nucleotide substitutions were predicted using computer analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The extent of functional disruption of the 11 <jats:italic>SLCO2A1<\/jats:italic> mutations identified in CEAS patients was variable, with 10 mutations (421GT, 547GA, 664GA, 770GA, 830dupT, 830delT, 940\u00a0+\u00a01GA, 1372GT, 1647GT, and 1807CT) resulting in loss or reduction of PGE<jats:sub>2<\/jats:sub> transport, excluding 97GC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PGE<jats:sub>2<\/jats:sub> transport ability of recombinant SLCO2A1 in <jats:styled-content style=\"fixed-case\"><jats:italic>X.\u00a0laevis<\/jats:italic><\/jats:styled-content> oocytes was hindered in 10\/11 <jats:italic>SLCO2A1<\/jats:italic> mutations identified in patients with CEAS. Further studies on the relationships between the different mutations and PGE<jats:sub>2<\/jats:sub> transport and clinical features, such as severity, are needed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15968","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T16:04:48Z","timestamp":1658765088000},"page":"1776-1784","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Functional analysis of mutant SLCO2A1 transporters found in patients with chronic enteropathy associated with <i>SLCO2A1<\/i>"],"prefix":"10.1111","volume":"37","author":[{"given":"Satowa","family":"Seki","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gen","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Hayashida","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Matsuura","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1178-3536","authenticated-orcid":false,"given":"Tadakazu","family":"Hisamatsu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2007.01.004"},{"key":"e_1_2_7_3_1","first-page":"1539","article-title":"Nonspecific multiple ulcer of the small intestine","volume":"3","author":"Okabe H","year":"1968","journal-title":"Stomach Intestine"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005581"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-006-9080-x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.111146"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201694660"},{"key":"e_1_2_7_8_1","first-page":"2121","article-title":"Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction","volume":"118","author":"Adler DH","year":"2008","journal-title":"J. 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