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Before EA intervention, they were divided into three groups: blank 1 group, blank 2 group, and IBS\u2010D model group. Diarrhea symptoms and visceral pain sensitivity were evaluated. After constructed the model successfully, the remaining IBS\u2010D model group rats were randomly divided into model group and EA group. Local intestinal inflammation (HE staining), changes of intestinal mucosa (occludin protein and microvascular diameter) were evaluated. Differences between two groups were compared using <jats:italic>t<\/jats:italic>\u2010test or Mann\u2013Whitney <jats:italic>U<\/jats:italic>\u2013test. Differences among more than two groups were compared using one\u2010way ANOVA or Kruskal\u2013Wallis test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After modeling, the results of HE staining in intestinal tract of IBS\u2010D model rats showed LGI. Compared with the model group, 4\u00a0h fecal moisture content (diarrhea index) and the AWR score were decreased in the EA group. The results of HE in EA group showed that the infiltration of intestinal inflammatory cells were alleviated. Additionally, EA significantly upregulated the expression of occludin protein and partially dilated the intestinal microvascular diameter. Pearson correlation analysis showed that the symptoms of IBS\u2010D rats were correlated with the changes of intestinal mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>EA may treat intestinal LGI in IBS\u2010D rats by upregulating the expression of occludin protein and dilating the intestinal microvascular diameter.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.15981","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T08:09:45Z","timestamp":1660291785000},"page":"1963-1974","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Ameliorating effects of electroacupuncture on the low\u2010grade intestinal inflammation in rat model of diarrhea\u2010predominant irritable bowel syndrome"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4120-0589","authenticated-orcid":false,"given":"Xuemei","family":"Li","sequence":"first","affiliation":[{"name":"Acupuncture and Tuina School\/The 3rd Teaching Hospital Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuiyu","family":"Ren","sequence":"additional","affiliation":[{"name":"Acupuncture and Tuina School\/The 3rd Teaching Hospital Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Hong","sequence":"additional","affiliation":[{"name":"Acupuncture and Tuina School\/The 3rd Teaching Hospital Chengdu University of Traditional Chinese Medicine  Chengdu China"},{"name":"Acupuncture and Chronobiology Key Laboratory of Sichuan Province  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Guo","sequence":"additional","affiliation":[{"name":"Acupuncture and Tuina School\/The 3rd Teaching Hospital Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuguang","family":"Yu","sequence":"additional","affiliation":[{"name":"Acupuncture and Tuina School\/The 3rd Teaching Hospital Chengdu University of Traditional Chinese Medicine  Chengdu China"},{"name":"Acupuncture and Chronobiology Key Laboratory of Sichuan Province  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0345-5740","authenticated-orcid":false,"given":"Sha","family":"Yang","sequence":"additional","affiliation":[{"name":"Acupuncture and Tuina School\/The 3rd Teaching Hospital Chengdu University of Traditional Chinese Medicine  Chengdu China"},{"name":"Acupuncture and Chronobiology Key Laboratory of Sichuan Province  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,23]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"507","article-title":"Effect of acupuncture at points in heel vessel for circadian clock genes of period 1 and period 2 mRNAs in the suprachiasmatic nucleus in insomnia rats","volume":"42","author":"Guo BJ","year":"2017","journal-title":"Zhen Ci Yan Jiu"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000001036"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/acupmed-2016-011320"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/acupmed-2015-010854"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2020.01.042"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i20.6044"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.608688"},{"key":"e_1_2_5_9_1","first-page":"605","article-title":"Efficacy and mechanism of acupuncture combined with Tongxieyaofang for diarrhea\u2010type irritable bowel syndrome of liver depression and spleen deficiency","volume":"40","author":"Wang SS","year":"2020","journal-title":"Zhongguo Zhen Jiu"},{"key":"e_1_2_5_10_1","first-page":"803","article-title":"Consensus of experts on irritable bowel syndrome in China in 2020","volume":"40","year":"2020","journal-title":"Chin J Dig"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2007.03399.x"},{"key":"e_1_2_5_12_1","first-page":"27","article-title":"Study on the shifting of Th1\/Th2 balance of large intestinal mucosa in patients with irritable bowel syndrome","author":"Li Y","year":"2004","journal-title":"Chin J Dig"},{"key":"e_1_2_5_13_1","first-page":"257","article-title":"Functional bowel disorders, are they really functional?","volume":"22","author":"Xiuli Z","year":"2017","journal-title":"Chin J Gastroenterol"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i14.3976"},{"key":"e_1_2_5_15_1","unstructured":"YangL.Effects of electroacupuncture on visceral sensitivity and intestinal microcirculation in IBS model rats. 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