{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:22:30Z","timestamp":1783455750156,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,5]],"date-time":"2022-11-05T00:00:00Z","timestamp":1667606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>Diagnosis of abdominal TB is an exigent task due to variable anatomical sites and non\u2010specific clinical manifestations that closely resemble other diseases. Most of the available diagnostic modalities yield low sensitivities and need expertise to handle the specialized equipment. Hence, there is an urgent need to develop a rapid and reliable diagnostic test, so as to reduce the unnecessary morbidity. Therefore, we designed a multi\u2010targeted loop\u2010mediated isothermal amplification (MT\u2010LAMP) for diagnosing abdominal TB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated an MT\u2010LAMP (using <jats:italic>mpt64<\/jats:italic> and IS<jats:italic>6110<\/jats:italic>) to diagnose abdominal TB within ascitic fluids and intestinal\/peritoneal biopsies and compared these results with multiplex\u2010PCR (M\u2010PCR) using the same targets. MT\u2010LAMP products were analyzed by gel electrophoresis and visual detection methods, that is, hydroxy naphthol blue and SYBR Green I reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sensitivities of 80.9% and 84.6% were obtained in suspected (<jats:italic>n<\/jats:italic>\u00a0=\u00a042) and total abdominal TB (<jats:italic>n<\/jats:italic>\u00a0=\u00a052) cases, respectively by gel\u2010based MT\u2010LAMP, with 97.3% (<jats:italic>n<\/jats:italic>\u00a0=\u00a037) specificity in non\u2010TB controls. Notably, sensitivities attained by gel\u2010based\/SYBR Green I MT\u2010LAMP in both clinically suspected and total abdominal TB cases were significantly higher (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) than M\u2010PCR. Furthermore, sensitivity obtained with SYBR Green I was equivalent to that of gel\u2010based MT\u2010LAMP, while somewhat lesser specificity (94.6%) was attained with SYBR Green I, compared with gel\u2010based MT\u2010LAMP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Both gel\u2010based and SYBR Green MT\u2010LAMP exhibited equivalent sensitivities to diagnose abdominal TB. Because SYBR Green LAMP is easier to perform than a gel\u2010based assay, we are currently focused on improving the specificity of this assay so as to develop a diagnostic kit.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.16036","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T16:31:23Z","timestamp":1666456283000},"page":"2264-2271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Diagnosis of abdominal tuberculosis by multi\u2010targeted (<i>mpt64<\/i> and IS<i>6110<\/i>) loop\u2010mediated isothermal amplification assay"],"prefix":"10.1111","volume":"37","author":[{"given":"Preeti","family":"Mor","sequence":"first","affiliation":[{"name":"Centre for Biotechnology Maharshi Dayanand University  Rohtak India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Sheoran","sequence":"additional","affiliation":[{"name":"Department of Statistics, Ramanujan College University of Delhi  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhawna","family":"Dahiya","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Maharshi Dayanand University  Rohtak India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev","family":"Parshad","sequence":"additional","affiliation":[{"name":"Department of General Surgery University of Health Sciences  Rohtak India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Nain","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia University of Health Sciences  Rohtak India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5442-3103","authenticated-orcid":false,"given":"Anish","family":"Khan","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Maharshi Dayanand University  Rohtak 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