{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T15:56:30Z","timestamp":1773330990380,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>In the absence of rapid on\u2010site evaluation (ROSE), it is not clear which method of tissue preparation is best to process tissue obtained from EUS guidance. Cytological smearing (CS), cell block (CB), and direct histology (DH) are the available techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To compare the diagnostic yield of three techniques of tissue preparation for EUS\u2010guided tissue acquisition without ROSE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients who were referred for EUS\u2010FNA of peri\u2010gastrointestinal masses were recruited. Without ROSE, each lesion was biopsied with three needle passes, and the order in which tissue is prepared was randomized to either (i) CS\u00a0+\u00a0CB, (ii) CB only, or (iii) DH only. The prepared specimens were reviewed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 243 specimens were taken from 81 patients. Tissue diagnosis was achieved in 78\/81 (96.3%) of patients, including 63 neoplasms (PDAC [<jats:italic>n<\/jats:italic>\u00a0=\u00a045], pancreatic neuroendocrine tumors [PNET; <jats:italic>n<\/jats:italic>\u00a0=\u00a04], cholangiocarcinoma [<jats:italic>n<\/jats:italic>\u00a0=\u00a05], metastatic disease [<jats:italic>n<\/jats:italic>\u00a0=\u00a04], lymphoma [<jats:italic>n<\/jats:italic>\u00a0=\u00a01], linitis plastica [<jats:italic>n<\/jats:italic>\u00a0=\u00a02], leiomyoma [<jats:italic>n<\/jats:italic>\u00a0=\u00a02]) and 15 benign pathologies (chronic pancreatitis [<jats:italic>n<\/jats:italic>\u00a0=\u00a08], reactive nodes [<jats:italic>n<\/jats:italic>\u00a0=\u00a05], inflammatory biliary stricture [<jats:italic>n<\/jats:italic>\u00a0=\u00a01], and pancreatic rest [<jats:italic>n<\/jats:italic>\u00a0=\u00a01]). The three non\u2010diagnostic cases were found to be PDAC (<jats:italic>n<\/jats:italic>\u00a0=\u00a02) and PNET (<jats:italic>n<\/jats:italic>\u00a0=\u00a01). Sensitivity and diagnostic accuracy was highest with DH (94 and 95%), which was significantly better than that by CS\u00a0+\u00a0CB (43 and 54%; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0001) and CB\u2010only preparations (32 and 48.6%; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001). There was no significant difference between the CS\u00a0+\u00a0CB and CB\u2010only arms (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.22).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Without ROSE, our findings suggest that with just a single pass, DH should be the tissue preparation method of choice given its significantly higher diagnostic accuracy compared with CS and\/or CB techniques.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.16137","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T18:14:33Z","timestamp":1675448073000},"page":"733-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Methods of tissue preparation after EUS\u2010guided tissue acquisition without rapid on\u2010site assessment: Results of a randomized 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Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua","family":"Zobel","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Royal Adelaide Hospital  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2122-8197","authenticated-orcid":false,"given":"Nam Q.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Royal Adelaide Hospital  Adelaide South Australia Australia"},{"name":"Discipline of Medicine University of Adelaide  Adelaide South Australia 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