{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T15:18:11Z","timestamp":1788016691659,"version":"build-2784847793"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T00:00:00Z","timestamp":1678060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>There are very few reports comparing the use of the University of Wisconsin solution and histidine\u2013tryptophan\u2013ketoglutarate solution as machine perfusion solutions for marginal liver grafts. We aimed to clarify whether the use of the histidine\u2013tryptophan\u2013ketoglutarate solution in hypothermic machine perfusion improves the split\u2010liver graft function in a large animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Porcine split\u2010liver grafts were created by 75% liver resection. Hypothermic machine perfusion experimental groups were divided as follows: Group 1, perfusate, University of Wisconsin gluconate solution (UW group; <jats:italic>n<\/jats:italic>\u00a0=\u00a05), and Group 2, perfusate, histidine\u2013tryptophan\u2013ketoglutarate solution (<jats:italic>HTK<\/jats:italic> group; <jats:italic>n<\/jats:italic>\u00a0=\u00a04). After 4\u00a0h of preservation, the liver function was evaluated using an isolated liver reperfusion model for 2\u00a0h.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the <jats:italic>HTK<\/jats:italic> group, the portal vein and hepatic artery resistance during hypothermic machine perfusion and the portal vein resistance during isolated liver reperfusion were lower than those in the UW group. In addition, the total Suzuki score for hepatic ischemia\u2013reperfusion injury in the <jats:italic>HTK<\/jats:italic> group was significantly better than that in the UW group. The number of anti\u2010ETS\u2010related genes staining\u2010positive sinusoid epithelial cell nuclei in the <jats:italic>HTK<\/jats:italic> group was higher than that in the UW group (not significant).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The histidine\u2013tryptophan\u2013ketoglutarate solution can be perfused with lower vascular resistance than the University of Wisconsin solution, reducing shear stress and preventing sinusoid epithelial cell injury in marginal grafts used as split\u2010liver grafts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jgh.16140","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T06:09:52Z","timestamp":1675750192000},"page":"783-790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Applicability of the histidine\u2013tryptophan\u2013ketoglutarate solution as a machine perfusion solution for marginal liver grafts"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3970-0827","authenticated-orcid":false,"given":"Hiroyoshi","family":"Iwata","sequence":"first","affiliation":[{"name":"Department of Transplantation Technology and Therapeutic Development Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5186-7331","authenticated-orcid":false,"given":"Naoto","family":"Matsuno","sequence":"additional","affiliation":[{"name":"Department of Transplantation Technology and Therapeutic Development Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6504-2197","authenticated-orcid":false,"given":"Daisuke","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Transplantation Technology and Therapeutic Development Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuka","family":"Toriumi","sequence":"additional","affiliation":[{"name":"Department of Transplantation Technology and Therapeutic Development Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3790-3011","authenticated-orcid":false,"given":"Masahide","family":"Otani","sequence":"additional","affiliation":[{"name":"Department of Transplantation Technology and Therapeutic Development Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuho","family":"Ohara","sequence":"additional","affiliation":[{"name":"Department of Transplantation Technology and Therapeutic Development Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0662-6138","authenticated-orcid":false,"given":"Hiromichi","family":"Obara","sequence":"additional","affiliation":[{"name":"Department of Mechanical System Engineering Tokyo Metropolitan University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4774-1936","authenticated-orcid":false,"given":"Yuji","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Department of Pathology Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6203-8528","authenticated-orcid":false,"given":"Hideki","family":"Yokoo","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic and Transplantation Surgery Asahikawa Medical University  Asahikawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01262776"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.5500\/wjt.v5.i3.89"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i33.7500"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.25843"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2009.02560.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.12659\/AOT.919920"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0b013e32835e29de"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2008.02165.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2007.01.003"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181fed021"},{"key":"e_1_2_7_12_1","first-page":"9","article-title":"Split\u2010liver ex situ machine perfusion: a novel technique for studying organ preservation and therapeutic interventions","author":"Huang V","year":"2020","journal-title":"J. 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