{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:51:39Z","timestamp":1791204699775,"version":"4.2.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,1,14]],"date-time":"2024-01-14T00:00:00Z","timestamp":1705190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,1,14]],"date-time":"2024-01-14T00:00:00Z","timestamp":1705190400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFC0906002"],"award-info":[{"award-number":["2016YFC0906002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFA0509200"],"award-info":[{"award-number":["2020YFA0509200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970718"],"award-info":[{"award-number":["31970718"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81830081"],"award-info":[{"award-number":["81830081"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972203"],"award-info":[{"award-number":["81972203"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070570"],"award-info":[{"award-number":["82070570"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82203224"],"award-info":[{"award-number":["82203224"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2022M712124"],"award-info":[{"award-number":["2022M712124"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"Abstract<\/jats:title>\n \n Background and Aim<\/jats:title>\n Patients with cholelithiasis (CL) or cholecystectomy (CE) would have more chances of getting colorectal adenoma (CRA) or cancer (CRC). We aimed to figure out the effects of gut microbiota and bile acid on colorectal neoplasm in CL and CE patients.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n This was a retrospective observational study that recruited 514 volunteers, including 199 people with normal gallbladders (normal), 152 CL, and 163 CE patients. Discovery cohort was established to explore the difference in gut microbiota through 16S rRNA and metagenomics sequencing. Validation cohort aimed to verify the results through quantitative polymerase chain reaction (qPCR).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Significant enrichment of\n \n Escherichia coli<\/jats:italic>\n <\/jats:styled-content>\n was found in patients with cholelithiasis or cholecystectomy both in the discovery cohort (16S rRNA sequencing,\n P<\/jats:italic>\n Normal\u2010CL<\/jats:sub>\n \u00a0=\u00a00.013,\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0=\u00a00.042; metagenomics sequencing,\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0=\u00a00.026) and validation cohort (\n P<\/jats:italic>\n Normal\u2010CL<\/jats:sub>\n \u00a0<\u00a00.0001,\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0<\u00a00.0001). Pks\n +<\/jats:sup>\n \n E.\u00a0coli<\/jats:italic>\n <\/jats:styled-content>\n was found enriched in CL and CE patients through qPCR (in discovery cohort:\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0=\u00a00.018; in validation cohort:\n P<\/jats:italic>\n Normal\u2010CL<\/jats:sub>\n \u00a0<\u00a00.0001,\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0<\u00a00.0001). The differences in bile acid metabolism were found both through Tax4Fun analysis of 16S rRNA sequencing (Ko00120, primary bile acid biosynthesis,\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0=\u00a00.014; Ko00121, secondary bile acid biosynthesis,\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0=\u00a00.010) and through metagenomics sequencing (map 00121,\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0=\u00a00.026). The elevation of serum total bile acid of CE patients was also found in validation cohort (\n P<\/jats:italic>\n Normal\u2010CE<\/jats:sub>\n \u00a0<\u00a00.0001). The level of serum total bile acid was associated with the relative abundance of pks\n +<\/jats:sup>\n \n E.\u00a0coli<\/jats:italic>\n <\/jats:styled-content>\n (\n r<\/jats:italic>\n \u00a0=\u00a00.1895,\n P<\/jats:italic>\n \u00a0=\u00a00.0012).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n \n E.\u00a0coli<\/jats:italic>\n <\/jats:styled-content>\n , especially pks\n +<\/jats:sup>\n species, was enriched in CL and CE patients. Pks\n +<\/jats:sup>\n \n E.\u00a0coli<\/jats:italic>\n <\/jats:styled-content>\n and bile acid metabolism were found associated with CRA and CRC in people after cholecystectomy.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jgh.16462","type":"journal-article","created":{"date-parts":[[2024,1,14]],"date-time":"2024-01-14T20:10:06Z","timestamp":1705263006000},"page":"868-879","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The effects of pks\n +<\/sup>\n \n Escherichia coli<\/i>\n <\/scp>\n and bile acid in colorectal tumorigenesis among people with cholelithiasis or cholecystectomy"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5630-1684","authenticated-orcid":false,"given":"Si\u2010Yuan","family":"Pan","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Institute of Digestive Disease Shanghai China"},{"name":"State Key, Laboratory for Oncogenes and Related Genes NHC Key Laboratory of Digestive Disease Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Bei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Institute of Digestive Disease Shanghai China"},{"name":"State Key, Laboratory for Oncogenes and Related Genes NHC Key Laboratory of Digestive Disease Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Wen","family":"Deng","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Institute of Digestive Disease Shanghai China"},{"name":"State Key, Laboratory for Oncogenes and Related Genes NHC Key Laboratory of Digestive Disease Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Lu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Institute of Digestive Disease Shanghai China"},{"name":"State Key, Laboratory for Oncogenes and Related Genes NHC Key Laboratory of Digestive Disease Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4302-9869","authenticated-orcid":false,"given":"Zhu\u2010Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Institute of Digestive Disease Shanghai China"},{"name":"State Key, Laboratory for Oncogenes and Related Genes NHC Key Laboratory of Digestive Disease Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2282-0248","authenticated-orcid":false,"given":"Jing\u2010Yuan","family":"Fang","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Institute of Digestive Disease Shanghai China"},{"name":"State Key, Laboratory for Oncogenes and Related Genes NHC Key Laboratory of Digestive Disease Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.03.005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-016-1289-7"},{"key":"e_1_2_6_4_1","first-page":"148","article-title":"The treatment of gallstone disease","volume":"117","author":"Gutt C","year":"2020","journal-title":"Dtsch. 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