{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:17:31Z","timestamp":1792034251379,"version":"4.4.0"},"reference-count":59,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T00:00:00Z","timestamp":1721260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"publisher","award":["232102310249"],"award-info":[{"award-number":["232102310249"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aim<\/jats:title>\n                    <jats:p>Nonalcoholic fatty liver disease (NAFLD) is currently one of the most common chronic liver diseases worldwide, characterized by the presence of lipid droplets. Rab18 is an important lipid droplet protein; however, its effects and mechanisms of action on NAFLD remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Free fatty acid\u2010stimulated AML\u201012 cells and high\u2010fat diet (HFD)\u2010fed mice were used as NAFLD models. Lentiviruses overexpressing Rab18 (Rab18\u2010OE) or knockdown (Rab18\u2010KD) were used to generate stable cell lines for genetic analysis. Blood serum levels of alanine aminotransferase, aspartate aminotransferase, total cholesterol, triglycerides, high\u2010density lipoprotein cholesterol, low\u2010density lipoprotein cholesterol, glucose, and leptin were measured using a biochemical autoanalyzer. Hematoxylin and eosin staining was performed to detect pathological damage to the liver. Lipid accumulation in the cells was assessed by Oil Red O staining. Target expression was measured using qPCR, western blotting, and immunocytochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Rab18 mRNA and protein expression levels increased in free fatty acid\u2010stimulated AML\u201012 cells and the livers of HFD\u2010fed mice. Rab18\u2010OE increased lipid accumulation\n                      <jats:italic>in vitro<\/jats:italic>\n                      , which was attenuated by Rab18\u2010KD.\n                      <jats:italic>In vivo<\/jats:italic>\n                      , Rab18\u2010OE augmented liver pathological damage, serum alanine aminotransferase\/aspartate aminotransferase activity, and triglyceride, total cholesterol, and low\u2010density lipoprotein levels, whereas Rab18\u2010KD decreased these indicators. Rab18\u2010KD also downregulated blood glucose levels in HFD\u2010fed mice. Mechanistically, Rab18\u2010OE and Rab18\u2010KD regulated the mRNA and protein expression levels of perilipin 2 (PLIN2) and peroxisome proliferator\u2010activated receptor gamma (PPAR\u03b3)\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      , respectively. Immunocytochemistry revealed that Rab18 colocalized with PLIN2 and PPAR\u03b3 in AML\u201012 cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Rab18 expression was elevated\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      in the NAFLD mouse model. Rab18 regulates PLIN2 and PPAR\u03b3 expression to exaggerate liver injury and lipid accumulation in patients with NAFLD. Thus, Rab18 may be a crucial protein in this disease and a potential therapeutic target.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jgh.16676","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T20:08:46Z","timestamp":1721333326000},"page":"2219-2227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Rab18 on liver injury and lipid accumulation by regulating perilipin 2 and peroxisome proliferator\u2010activated receptor gamma in non\u2010alcoholic fatty liver disease"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1730-2590","authenticated-orcid":false,"given":"Lei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Zhengzhou Central Hospital Affiliated to Zhengzhou University  Zhengzhou 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