{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:36:25Z","timestamp":1778668585566,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100022963","name":"Key Research and Development Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2022C03108"],"award-info":[{"award-number":["2022C03108"]}],"id":[{"id":"10.13039\/100022963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1100500"],"award-info":[{"award-number":["2021YFA1100500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aim<\/jats:title>\n                    <jats:p>Hepatic ischemia\u2013reperfusion (I\/R) injury is the primary cause of liver dysfunction and liver failure, commonly occurring in liver transplantation, hepatectomy, and hemorrhagic shock. Polo\u2010like kinase 2 (PLK2), a pivotal regulator of centriole duplication, plays a crucial role in cell proliferation and injury repair. However, the function of PLK2 in hepatic I\/R remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The effect of PLK2 was investigated in the mouse hepatic I\/R model and the hepatocyte hypoxia\u2010reoxygenation (H\/R) model. Liver injury was assessed by serum transaminase and hematoxylin and eosin staining. Cell apoptosis was analyzed using TUNEL analysis and immunoblotting. Inflammatory factors were evaluated by reverse transcription\u2010quantitative polymerase chain reaction. Mice or cultured cells during the I\/R or H\/R were treated by overexpressing PLK2. ROS fluorescence staining was used to assess oxidative stress injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PLK2 was upregulated after hepatic I\/R injury. Overexpressed PLK2 significantly improved liver enzyme levels and alleviated liver histological injury. Moreover, PLK2 decreased hepatocyte apoptosis and inhibited the expression of inflammatory factors in liver. Mechanistically, PLK2 increased the phosphorylation of GSK3\u03b2 and enhanced expression of the antioxidant enzyme HO\u20101, leading to less ROS production. Inhibition of the HO\u20101 aggravated ROS generation and abolished the protective effect of PLK2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, these findings revealed that PLK2 enhanced HO\u20101 expression and reduced oxidative stress damage in hepatic I\/R injury, and this protective effect related to GSK3\u03b2 activity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jgh.16815","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T01:33:57Z","timestamp":1732066437000},"page":"304-314","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["PLK2 inhibited oxidative stress and ameliorated hepatic ischemia\u2013reperfusion injury through phosphorylating GSK3\u03b2"],"prefix":"10.1111","volume":"40","author":[{"given":"Wenwen","family":"Ge","sequence":"first","affiliation":[{"name":"Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhoucheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyang","family":"Zhong","sequence":"additional","affiliation":[{"name":"Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutong","family":"Chen","sequence":"additional","affiliation":[{"name":"The Fourth School of Clinical Medicine Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Tang","sequence":"additional","affiliation":[{"name":"Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shusen","family":"Zheng","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Combined Multi\u2010Organ Transplantation Zhejiang University  Hangzhou China"},{"name":"Department of Hepatobiliary and Pancreatic Surgery Shulan (Hangzhou) Hospital  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2761-2811","authenticated-orcid":false,"given":"Xiao","family":"Xu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Combined Multi\u2010Organ Transplantation Zhejiang University  Hangzhou China"},{"name":"Department of Hepatobiliary and Pancreatic Surgery and Minimally Invasive Surgery, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), School of Clinical Medicine Hangzhou Medical College  Hangzhou China"},{"name":"Institute of Translational Medicine Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4152-8752","authenticated-orcid":false,"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery and Minimally Invasive Surgery, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), School of Clinical Medicine Hangzhou Medical College  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2011.03579.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2507"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-308513"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2024.102033"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1785"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.01.013"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2653"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.19.6936-6943.2003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.319425"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102394"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22815"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.11.029"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1241-16.2016"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2020-0361"},{"key":"e_1_2_6_16_1","article-title":"Management of ROS and regulatory cell death in myocardial ischemia\u2010reperfusion injury","volume":"9","author":"Gong G","year":"2024","journal-title":"Mol. Biotechnol."},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.22157"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101275"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2019.104720"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2013.06.017"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01688-x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.6064\/2012\/513192"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2014.05.022"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13116"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.25779"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2011.00040"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.31335"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.05124.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2008.06.007"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9FO01808E"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.02.012"}],"updated-by":[{"DOI":"10.1111\/jgh.16966","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000}}],"container-title":["Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.16815","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jgh.16815","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.16815","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T01:14:09Z","timestamp":1771204449000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jgh.16815"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,19]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/jgh.16815"],"URL":"https:\/\/doi.org\/10.1111\/jgh.16815","archive":["Portico"],"relation":{},"ISSN":["0815-9319","1440-1746"],"issn-type":[{"value":"0815-9319","type":"print"},{"value":"1440-1746","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,19]]},"assertion":[{"value":"2024-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}