{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:27:03Z","timestamp":1787776023431,"version":"build-2784847793"},"reference-count":102,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["111\u20102314\u2010B\u2010002\u2010198\u2010MY3"],"award-info":[{"award-number":["111\u20102314\u2010B\u2010002\u2010198\u2010MY3"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Gastrointestinal (GI) cancers represent a significant global health burden, being among the leading causes of cancer\u2010related deaths. The prognosis for patients remains unsatisfactory, largely because most cancers are detected at advanced stages. Traditional diagnostic methods, such as radiological and histopathological examinations and serum tumor markers like AFP, CEA, CA\u2010125, and CA\u2010199, possess limitations in sensitivity and specificity, particularly for early screening. A major drawback of tissue biopsy is its inability to fully capture the inherent heterogeneity within tumors, as mutations can differ between primary and metastatic sites. In this context, liquid biopsy has emerged as a promising, minimally invasive alternative for detecting cancer\u2010associated materials present in various body fluids. The concept of liquid biopsy, initially centered on circulating tumor cells, has expanded to encompass other critical biomarkers such as circulating tumor DNA, extracellular vesicles, and circulating tumor RNA. Analyzing these biomarkers using advanced techniques like next\u2010generation sequencing or proteomics can unveil a wealth of potential information. Liquid biopsy offers numerous advantages, being less invasive, more convenient, potentially more cost\u2010effective, and providing a dynamic, real\u2010time snapshot of the entire tumor burden that reflects both intertumoral and intratumoral heterogeneity. This review provides an overview of key liquid biopsy biomarkers and their associated detection technologies, discusses their burgeoning clinical applications across various GI cancer types, and highlights the current challenges and future directions in this rapidly evolving field.<\/jats:p>","DOI":"10.1111\/jgh.70219","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:33:13Z","timestamp":1768969993000},"page":"546-555","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Novel Liquid Biopsy in Gastrointestinal Cancers"],"prefix":"10.1111","volume":"41","author":[{"given":"Li\u2010Chun","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Internal Medicine National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tang\u2010Long","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology (PPM) and International Joint Degree Master's Program in Agro\u2010Biomedicine in Food and Health (NTU GIP\u2010TRIAD) National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5325-3974","authenticated-orcid":false,"given":"Takahiro","family":"Ochiya","sequence":"additional","affiliation":[{"name":"Division of Molecular and Cellular Medicine, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.74.2049"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdz227"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21388"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2019.100839"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01715-z"},{"key":"e_1_2_12_7_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41571-019-0279-0","article-title":"Liquid Outperforms Tissue","volume":"16","author":"Romero D.","year":"2019","journal-title":"Nature Reviews. Clinical Oncology"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2010.07.001"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01556-2"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-16-1000"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-43139-0_5"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.21037\/jgo.2016.08.08"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0561-9"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8250"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12507"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.21147\/j.issn.1000-9604.2019.06.10"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.01.011"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2015.1091729"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2531-z"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33217"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23147588"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvab204"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1200\/OP.24.00167"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-021-00562-2"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2018.1400384"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-21-1982"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.125"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2019-318860"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v17.i4.103591"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4591"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10147-017-1104-3"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2024.101668"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.4c03869"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1643670"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b08199"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c13557"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2025.113877"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.679366"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-95685-5"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.107773"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.732743"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.02.027"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.4103\/jcrt.jcrt_1212_22"},{"key":"e_1_2_12_44_1","first-page":"1088","article-title":"Screening of Early\u2010Staged Colorectal Neoplasia by Clonal Hematopoiesis\u2010Based Liquid Biopsy and Machine\u2010Learning","volume":"12","author":"Hsu Y. C.","year":"2022","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-018-0559-5"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-023-10488-y"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2024-333657"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep4.1451"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322065"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2020.02.011"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-016-0087-0"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180251"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-008-0122-9"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.769203"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2780-8_15"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0217586"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.3755"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12907-018-0079-y"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2018-316522"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-025-02515-1"},{"key":"e_1_2_12_61_1","first-page":"1828","article-title":"Mining Predictive Biomarker for Neoadjuvant Chemotherapy in Gastric Cancer by Proteomics","volume":"58","author":"Qian J.","year":"2011","journal-title":"Hepato\u2010Gastroenterology"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdz390"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2304714"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2025.7515"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-018-0382-z"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.esmogo.2025.100140"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v5.31488"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031379"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3511"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b07782"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13085"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2020.1809765"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2024.5635"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1080\/1750743X.2024.2430941"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241210230"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-24-2396"},{"key":"e_1_2_12_77_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2019.0528"},{"key":"e_1_2_12_78_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2023.02.044"},{"key":"e_1_2_12_79_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-019-01601-w"},{"key":"e_1_2_12_80_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S494747"},{"key":"e_1_2_12_81_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/9635218"},{"key":"e_1_2_12_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-79252-y"},{"key":"e_1_2_12_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24341"},{"key":"e_1_2_12_84_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15500"},{"key":"e_1_2_12_85_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2024.0345"},{"key":"e_1_2_12_86_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2024.1575"},{"key":"e_1_2_12_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.120105"},{"key":"e_1_2_12_88_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-025-05669-x"},{"key":"e_1_2_12_89_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c04428"},{"key":"e_1_2_12_90_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-025-01681-3"},{"key":"e_1_2_12_91_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-016-2526-4"},{"key":"e_1_2_12_92_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-023-13126-x"},{"key":"e_1_2_12_93_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djac027"},{"key":"e_1_2_12_94_1","article-title":"Cost\u2010Effectiveness of Novel Noninvasive Screening Tests for Colorectal Neoplasia","author":"Shaukat A.","year":"2025","journal-title":"Clinical Gastroenterology and Hepatology"},{"key":"e_1_2_12_95_1","doi-asserted-by":"publisher","DOI":"10.7326\/ANNALS-24-00910"},{"key":"e_1_2_12_96_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2024.03.011"},{"key":"e_1_2_12_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2025.04.003"},{"key":"e_1_2_12_98_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-021-01471-y"},{"key":"e_1_2_12_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2020.06.007"},{"key":"e_1_2_12_100_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-22-2538"},{"key":"e_1_2_12_101_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-016-9879-z"},{"key":"e_1_2_12_102_1","first-page":"12991","article-title":"Identification of Colorectal Cell Exosomes by Raman Spectral Analysis","volume":"11","author":"Weiwei Zheng W. H.","year":"2018","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"e_1_2_12_103_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-021-01431-6"}],"container-title":["Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.70219","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jgh.70219","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.70219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T19:09:20Z","timestamp":1781809760000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jgh.70219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,20]]},"references-count":102,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/jgh.70219"],"URL":"https:\/\/doi.org\/10.1111\/jgh.70219","archive":["Portico"],"relation":{},"ISSN":["0815-9319","1440-1746"],"issn-type":[{"value":"0815-9319","type":"print"},{"value":"1440-1746","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,20]]},"assertion":[{"value":"2025-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}