{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T09:52:08Z","timestamp":1775641928642,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Intrahepatic cholangiocarcinoma (ICC), a primary liver malignancy arising from the intrahepatic bile ducts, represents one of the most aggressive tumors with a dismal prognosis and ranks second only to hepatocellular carcinoma (HCC) in prevalence. Dysregulation of phospholipid metabolism is increasingly being implicated in tumorigenesis, and lysophosphatidylcholine acyltransferase (LPCAT) family members\u2014key regulatory enzymes\u2014play critical roles in various cancers, including HCC. However, their expression profiles and biological functions in ICC remain undefined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunochemistry experiments were used to examine the expression of LPCAT1 in ICC tissues. Both in\u00a0vitro and in\u00a0vivo functional assays were performed to investigate the functional role of LPCAT1 in regulating cell proliferation, apoptosis, and tumor growth. RNA sequencing and multiplex immunohistochemistry were used to explore the signaling pathways regulated by LPCAT1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified LPCAT1 as the most significantly upregulated LPCAT in both human and murine ICC tissues. Elevated LPCAT1 expression correlated with poor prognosis in ICC patients. Genetic silencing or overexpression of LPCAT1 dramatically altered the proliferation capacity of ICC cells both in\u00a0vitro and in\u00a0vivo. Furthermore, liver\u2010specific LPCAT1 knockout mice (LPCAT1\n                      <jats:sup>LCKO<\/jats:sup>\n                      ) exhibited significantly attenuated liver tumor burden in a chemically induced model of ICC. Combined immunoblot and RNA sequencing analyses revealed that LPCAT1 upregulates the expression of cell cycle proteins through activation of the PI3K\/AKT signaling pathway, thereby promoting cellular proliferation and accelerating ICC progression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>LPCAT1 upregulates cell cycle proteins through activation of the PI3K\/AKT signaling pathway, thereby promoting cellular proliferation and accelerating ICC progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jgh.70291","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T12:33:53Z","timestamp":1771504433000},"page":"1267-1279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oncogenic LPCAT1 Accelerates Intrahepatic Cholangiocarcinoma Development Through PI3K\/Akt Signaling\u2010Dependent Cell Cycle Regulation"],"prefix":"10.1111","volume":"41","author":[{"given":"Enlai","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of General Surgery, Xinqiao Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Institute of Clinical Pathology, Key Laboratory of Transplant Engineering and Immunology, NHC, West China Hospital Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Menglin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Institute of Clinical Pathology, Key Laboratory of Transplant Engineering and Immunology, NHC, West China Hospital Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Liver Transplantation Center, West China Hospital Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daofeng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Xinqiao Hospital Army Medical University  Chongqing China"},{"name":"Department of General Surgery, Liver Transplantation Center, West China Hospital Sichuan University  Chengdu China"},{"name":"Lead Contract"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6885-9392","authenticated-orcid":false,"given":"Qing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Xinqiao Hospital Army Medical University  Chongqing China"},{"name":"Department of Pathology and Institute of Clinical 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