{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:58:47Z","timestamp":1790405927402,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gallstone disease is increasingly recognized as involving host\u2013microbe\u2013metabolite interactions. Although biliary microbial communities have been detected in biliary obstruction, their relationship with bile acid remodeling in common bile duct (CBD) stone disease remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>ERCP\u2010acquired bile samples from 25 patients, including 12 with CBD stones and 13 patients with non\u2010stone pancreatobiliary diseases, including malignant and benign conditions, were analyzed using 16S rRNA sequencing and targeted bile acid metabolomics. Between\u2010group comparisons and exploratory microbe\u2013bile acid correlation analyses were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bile samples contained detectable microbial communities dominated by\n                      <jats:italic>Pseudomonadota<\/jats:italic>\n                      and\n                      <jats:italic>Bacillota<\/jats:italic>\n                      . Alpha diversity and overall community structure were comparable between groups, with no significant beta\u2010diversity separation (PERMANOVA\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.0483,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.259). No taxa remained significant after multiple\u2010testing correction, although several genera showed nominal between\u2010group differences. Targeted metabolomics showed only mild separation between groups. TUDCA\/THDCA and UDCA were higher in the CBD stone group at the nominal\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05 level, whereas 12\u2010O\u2010CDCA showed a nonsignificant increasing trend; none remained significant after FDR correction. Exploratory multi\u2010omics analysis suggested nominal microbe\u2013bile acid associations, but these correlations were also not significant after FDR correction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This pilot multi\u2010omics study identified detectable biliary microbial communities and exploratory bile acid remodeling trends in ERCP\u2010acquired bile from patients with CBD stones. However, key taxonomic, metabolomic, and association findings did not survive multiple\u2010testing correction and should be interpreted as hypothesis\u2010generating observations requiring validation in larger, well\u2010characterized cohorts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jgh.70776","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:12:01Z","timestamp":1790403121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biliary Microbiota and Bile Acid Remodeling in Common Bile Duct Stone Patients: A Pilot Multi\u2010Omics Analysis of ERCP\u2010Acquired Human Bile"],"prefix":"10.1111","author":[{"given":"Wenxin","family":"Xia","sequence":"first","affiliation":[{"name":"The Second Clinical Medical College Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaolin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hangzhou Red Cross Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8368-9564","authenticated-orcid":false,"given":"Yutao","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hangzhou Red Cross Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilong","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery and Thyroid &amp; Breast Surgery Hangzhou Red Cross Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8548-9869","authenticated-orcid":false,"given":"Dongya","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hangzhou Red Cross Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hangzhou Red Cross Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.l5709"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.08.025"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201014\u2010669"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201027758\u20108"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010019\u20100712\u20108"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1131694"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010022\u201000805\u2010x"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01296423"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2021.36.e94"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620\u2010020\u201006173\u2010x"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440\u20101746.2009.06072.x"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150519"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11082124"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.625589"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.synbio.2021.11.002"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.5009\/gnl20214"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462\u20105822.2003.00310.x"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201071610\u20100"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.05.030"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9091900"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26020612"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.1.193\u2010200.1999"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867324666170530080636"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R900011\u2010JLR200"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010016\u20108006\u20106"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0000000000000057"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S557300"}],"container-title":["Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.70776","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jgh.70776","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jgh.70776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:12:03Z","timestamp":1790403123000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jgh.70776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":27,"alternative-id":["10.1111\/jgh.70776"],"URL":"https:\/\/doi.org\/10.1111\/jgh.70776","archive":["Portico"],"relation":{},"ISSN":["0815-9319","1440-1746"],"issn-type":[{"value":"0815-9319","type":"print"},{"value":"1440-1746","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]},"assertion":[{"value":"2026-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jgh.70776"}}