{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:55:01Z","timestamp":1792050901403,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n This study investigates the relationship between gut microbiota dysbiosis and chronic constipation (CC) and evaluates the therapeutic efficacy of fecal microbiota transplantation (FMT) in restoring microbial homeostasis and metabolic function.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We retrospectively analyzed existing before\u2010after clinical data from 206 patients with CC who received FMT. No placebo or case\u2013control group was included. Gut microbiota profiles were analyzed before and after FMT using 16S rRNA sequencing. Alpha and beta diversity indices were calculated to assess microbial community evolution. Gas chromatography\u2013mass spectrometry (GC\u2013MS) was employed to quantify short\u2010chain fatty acids (SCFAs). Patients were stratified into butyrate\u2010high and butyrate\u2010low metabolic subtypes using a partitioning around medoids (PAM) algorithm based on pre\u2010transplant butyrate levels. Clinical severity was independently defined using Rome IV criteria, CSBM, BSFS, and PAC\u2010SYM.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n FMT significantly increased microbial \u03b1\u2010diversity and shifted the community structure toward that of healthy donors. Post\u2010transplant, the\n Bacteroidetes<\/jats:italic>\n \/\n Firmicutes<\/jats:italic>\n ratio decreased from 46.8% to 38.9%, with a marked enrichment of beneficial taxa such as\n Faecalibacterium<\/jats:italic>\n ,\n Akkermansia<\/jats:italic>\n , and\n Bifidobacterium<\/jats:italic>\n . These shifts correlated with a significant rise in SCFA concentrations, particularly acetic and butyric acids. Butyrate deficiency was characterized by broader SCFA deficiencies and fragmented microbial networks. Multivariable binary logistic regression identified\n Faecalibacterium<\/jats:italic>\n , ACE index, and acetic acid as independent predictors of clinically defined constipation severity.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n FMT was associated with increased microbial diversity, restoration of SCFA\u2010producing taxa, and increased SCFA concentrations in patients with CC. These changes accompanied improvements in CSBM, BSFS, PAC\u2010SYM, and PAC\u2010QOL.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jgh.70807","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:34:50Z","timestamp":1792049690000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of Gut Microbiota in Chronic Constipation and the Therapeutic Potential of Fecal Microbiota Transplantation"],"prefix":"10.1111","author":[{"given":"Xinxin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pelvic Floor Disorders Treatment Center The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pelvic Floor Disorders Treatment Center The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8307-9683","authenticated-orcid":false,"given":"Yuedong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anorectal The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02023-24","article-title":"The Impact of Small Intestinal Bacterial Overgrowth on the Efficacy of Fecal Microbiota Transplantation in Patients With Chronic Constipation","volume":"15","author":"Wang L.","year":"2024","journal-title":"MBio"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1007\/s11427-017-9220-7","article-title":"Microbial Treatment in Chronic Constipation","volume":"61","author":"Huang L.","year":"2018","journal-title":"Science China. 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