{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:59:48Z","timestamp":1790218788435,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,5,27]],"date-time":"2016-05-27T00:00:00Z","timestamp":1464307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571503"],"award-info":[{"award-number":["81571503"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300556"],"award-info":[{"award-number":["81300556"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Human Nutrition Diet"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Being small for gestational age (<jats:styled-content style=\"fixed-case\">SGA<\/jats:styled-content>), a foetal growth abnormality, has a long\u2010lasting impact on childhood health. Its aetiology and underlying mechanisms are not well understood. Underlying epigenetic changes of imprinted genes have emerged as a potential pathological pathway because they may be associated with growth, including <jats:styled-content style=\"fixed-case\">SGA<\/jats:styled-content>. As a common methyl donor, folic acid (<jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>) is essential for <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation, synthesis and repair, and <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content> supplementation is widely recommended for women planning pregnancy. The present study aimed to investigate the inter\u2010relationships among methylation levels of two imprinted genes [<jats:italic>H19<\/jats:italic> differentially methylated regions (<jats:styled-content style=\"fixed-case\">DMR<\/jats:styled-content>s) and <jats:italic><jats:styled-content style=\"fixed-case\">MEST<\/jats:styled-content><\/jats:italic> <jats:styled-content style=\"fixed-case\">DMR<\/jats:styled-content>s], maternal <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content> supplementation and <jats:styled-content style=\"fixed-case\">SGA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted a case\u2013control study. Umbilical cord blood was taken from 39 <jats:styled-content style=\"fixed-case\">SGA<\/jats:styled-content> infants and 49 controls whose birth weights are appropriate for gestational age (<jats:styled-content style=\"fixed-case\">AGA<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation levels of <jats:italic>H19<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">MEST<\/jats:styled-content><\/jats:italic> <jats:styled-content style=\"fixed-case\">DMR<\/jats:styled-content>s were determined by an analysis of mass array quantitative methylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Statistically significantly higher methylation levels were observed at sites 7.8, 9 and 17.18 of <jats:italic>H19<\/jats:italic> (<jats:italic>P<\/jats:italic> = 0.030, 0.016 and 0.050, respectively) in the <jats:styled-content style=\"fixed-case\">SGA<\/jats:styled-content> infants compared to the <jats:styled-content style=\"fixed-case\">AGA<\/jats:styled-content> group. In addition, the association was stronger in male births where the mothers took <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content> around conception at six <jats:italic>H19<\/jats:italic> sites (<jats:italic>P<\/jats:italic> = 0.004, 0.005, 0.048, 0.002, 0.021 and 0.005, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Methylation levels at <jats:italic>H19 <\/jats:italic><jats:styled-content style=\"fixed-case\">DMR<\/jats:styled-content>s were higher in <jats:styled-content style=\"fixed-case\">SGA<\/jats:styled-content> infants compared to <jats:styled-content style=\"fixed-case\">AGA<\/jats:styled-content> controls. It appears that the association may be influenced by maternal peri\u2010conception <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content> supplementation and also be sex\u2010specific.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jhn.12369","type":"journal-article","created":{"date-parts":[[2016,5,27]],"date-time":"2016-05-27T05:35:04Z","timestamp":1464327304000},"page":"643-651","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Effects of maternal folic acid supplementation on gene methylation and being small for gestational age"],"prefix":"10.1111","volume":"29","author":[{"given":"Y.\u2010Y.","family":"Qian","sequence":"first","affiliation":[{"name":"Shanghai Medical College of Fudan University Shanghai China"},{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.\u2010L.","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai First Maternity and Infant Hospital Tongji University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.\u2010F.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010H.","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010P.","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders Bio\u2010X Institutes Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology School of Life Sciences Fudan University Shanghai China"},{"name":"Institutes of Biomedical Sciences Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u2010H.","family":"Miao","sequence":"additional","affiliation":[{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Du","sequence":"additional","affiliation":[{"name":"Key Lab. of Reproduction Regulation of NPFPC SIPPR IRD Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.\u2010K.","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Research Kaiser Permanente Oakland CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,27]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"59","article-title":"Comparison between two growth curves for small for gestational age diagnosis","volume":"37","author":"Rodrigues FP","year":"2015","journal-title":"Rev Bras Ginecol Obstet"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.11112"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/db08-0558"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2008.00904.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2014-005388"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20522"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1203\/pdr.0b013e318045bedb"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-15-67"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-472"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.140077mb"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.6.2.13516"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.10958"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/msj.20176"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu290"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-006-9096-3"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.2012.01844.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2006.042135"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501749200"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1997.4731"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2007.04.013"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0328"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/35047554"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2015.01.002"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.6.7.16263"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040924"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.53.3-4.341"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.07.074"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb05972.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2015.03.007"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/138.4.703"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0321006"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114507819131"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.11.949"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13372-7"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201200186"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/83.5.993"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph10094352"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/129.4.779"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1159\/000301555"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.5.781"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.11.1907"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.04.021"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/2464"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200900170"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00061.2014"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24225"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/CEJ.0b013e328348fc21"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.03.038"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.08.027"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddm010"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1017\/S204017441300041X"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.04.025"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081731"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.20734"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.2337\/db12-0289"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.12.002"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20027"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-11-29"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/1868-7083-3-2"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi.13.24"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2015.04.009"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.012393"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114509288994"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2008.01.010"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007845"}],"container-title":["Journal of Human Nutrition and Dietetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjhn.12369","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjhn.12369","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jhn.12369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T13:48:22Z","timestamp":1696168102000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jhn.12369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,27]]},"references-count":65,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1111\/jhn.12369"],"URL":"https:\/\/doi.org\/10.1111\/jhn.12369","archive":["Portico"],"relation":{},"ISSN":["0952-3871","1365-277X"],"issn-type":[{"value":"0952-3871","type":"print"},{"value":"1365-277X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,27]]},"assertion":[{"value":"2016-05-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}