{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T08:25:17Z","timestamp":1769156717076,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T00:00:00Z","timestamp":1430265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Invest & Clin Dent"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>To quantify the level of serum malondialdehyde and extent of DNA<\/jats:styled-content> damage using comet assay in patients with oral submucous fibrosis (SMF<\/jats:styled-content>) in comparison to normal individuals and to correlate the extent of DNA<\/jats:styled-content> damage with MDA<\/jats:styled-content> levels.<\/jats:p><\/jats:sec>Methods<\/jats:title>Study included 30 cases of SMF<\/jats:styled-content> (n<\/jats:italic>\u00a0=\u00a030) and equal number of healthy volunteers. Serum malondialdehyde was measured using the thiobarbituric\u2013trichloroacetitic acid (TBA<\/jats:styled-content>\u2013TCA<\/jats:styled-content>) method. Comet assay was used to assess the DNA<\/jats:styled-content> damage. Association between the extent of DNA<\/jats:styled-content> damage and serum MDA<\/jats:styled-content> levels was analyzed in SMF<\/jats:styled-content> statistically.<\/jats:p><\/jats:sec>Results<\/jats:title>Comet assay results showed that there was an increase in tail length, percentage of tail DNA<\/jats:styled-content> and tail moment among SMF<\/jats:styled-content> subjects (P<\/jats:italic>\u00a0<\u00a00.05). Serum MDA<\/jats:styled-content> levels were elevated in SMF<\/jats:styled-content> patients compared with healthy subjects. A significant positive correlation was observed between serum MDA<\/jats:styled-content> levels and comet tail length in SMF<\/jats:styled-content> group (r<\/jats:italic>\u00a0=\u00a00.56; P<\/jats:italic>\u00a0<\u00a00.05).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Patients with SMF<\/jats:styled-content> have increased DNA<\/jats:styled-content> damage and elevated levels of lipid peroxidation compared with healthy controls. Evaluation of MDA<\/jats:styled-content> levels as an oxidative biomarker along with comet assay analysis will serve as a diagnostic tool to identify patients with high risk of malignant potential in SMF<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jicd.12153","type":"journal-article","created":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T09:11:08Z","timestamp":1430298668000},"page":"286-293","source":"Crossref","is-referenced-by-count":13,"title":["Estimation of serum malondialdehyde and assessment of DNA damage using comet assay in patients with oral submucous fibrosis"],"prefix":"10.1111","volume":"7","author":[{"given":"Swetha","family":"Paulose","sequence":"first","affiliation":[{"name":"Department of Oral Medicine and Radiology Indira Gandhi Institute of Dental Sciences Sri Balaji Vidyapeeth University Pondicherry 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