{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T03:57:26Z","timestamp":1778126246753,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T00:00:00Z","timestamp":1540771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Invest & Clin Dent"],"published-print":{"date-parts":[[2019,2]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>The aim of the present study was to determine and compare the expression profile of periostin (POSTN<\/jats:styled-content><\/jats:italic>), sclerostin (SOST<\/jats:styled-content><\/jats:italic>), receptor activator nuclear factor\u2010\u03baB (RANK<\/jats:styled-content><\/jats:italic>), and RANK ligand (RANKL<\/jats:styled-content><\/jats:italic>) genes in gingival tissue samples collected from healthy gingiva (control) and severe chronic periodontitis sites.<\/jats:p><\/jats:sec>Methods<\/jats:title>Fifty systemically\u2010healthy individuals was enrolled in the present case\u2010control study. Gingival tissue samples were obtained from healthy gingiva (N\u00a0=\u00a025) and sites with severe chronic periodontitis (N\u00a0=\u00a025). Total RNA<\/jats:styled-content> was isolated from all the tissues. cDNA<\/jats:styled-content> conversion was then performed using a reverse transcription polymerase chain reaction (PCR)<\/jats:styled-content> program. Real\u2010time PCR and SYBR<\/jats:styled-content> green method were used to determine the expression levels of SOST<\/jats:styled-content><\/jats:italic>, POSTN<\/jats:styled-content><\/jats:italic>, RANK<\/jats:styled-content><\/jats:italic>, and RANKL<\/jats:styled-content><\/jats:italic> genes.<\/jats:p><\/jats:sec>Results<\/jats:title>An elevated expression (3.5\u20104\u2010fold) of SOST<\/jats:styled-content><\/jats:italic>, RANK<\/jats:styled-content><\/jats:italic>, and RANKL<\/jats:styled-content><\/jats:italic> genes, with a concomitant reduced expression of the POSTN<\/jats:styled-content><\/jats:italic> gene, was identified in severe chronic periodontitis. The intergroup difference between the mean delta cyclic threshold values showed statistical significance at P<\/jats:italic><.001.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The expression profile of SOST<\/jats:styled-content><\/jats:italic>, RANK<\/jats:styled-content><\/jats:italic>, RANKL<\/jats:styled-content><\/jats:italic>, and POSTN<\/jats:styled-content><\/jats:italic> genes observed in gingival tissue samples from sites with severe chronic periodontitis and healthy gingiva suggests that the differential level of the gene expression could serve as an indicator of periodontitis progression\/severity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jicd.12369","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T06:28:36Z","timestamp":1540880916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Differential expression of periostin, sclerostin, receptor activator of nuclear factor\u2010\u03baB, and receptor activator of nuclear factor\u2010\u03baB ligand genes in severe chronic periodontitis"],"prefix":"10.1111","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0195-0476","authenticated-orcid":false,"given":"Pooja A.","family":"Sankardas","sequence":"first","affiliation":[{"name":"Department of Periodontology Faculty of Dental Sciences Sri Ramachandra University Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vamsi","family":"Lavu","sequence":"additional","affiliation":[{"name":"Department of Periodontology Faculty of Dental Sciences Sri Ramachandra University Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhaskar V. 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