{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T23:11:38Z","timestamp":1763766698247,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T00:00:00Z","timestamp":1553644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Invest &amp; Clin Dent"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Evidence of glucose supplementation effect on <jats:italic>Candida<\/jats:italic> biofilm metabolism has not been demonstrated. Therefore, the aim of the present study was to evaluate the effect of glucose concentration on <jats:italic>Candida<\/jats:italic> biofilms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Single\u2010 and dual\u2010species biofilms of <jats:italic>Candida<\/jats:italic> were grown on saliva\u2010coated poly(methyl\u2010methacrylate) disks for 72\u00a0hours. Biofilms (N\u00a0=\u00a08\/group) were exposed to the following concentrations of glucose: 100\u00a0mmol\/L (G100), 300\u00a0mmol\/L (G300), and no glucose (G0: control). Biofilms were collected to determine the acidogenicity, viability, amount of soluble and insoluble extracellular polysaccharides (<jats:styled-content style=\"fixed-case\">IEPS<\/jats:styled-content>), and surface roughness. Data were analyzed using analysis of variance and Tukey's tests (\u03b1\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Single\u2010 and dual\u2010species biofilms from G300 were more acidogenic at 48 and 72\u00a0hours compared to G100 and G0 (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). The viability of the G100 and G300 groups did not differ (<jats:italic>P\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>0.05), but differed statistically from G0. The amount of <jats:styled-content style=\"fixed-case\">IEPS<\/jats:styled-content> in the G300 group was statistically higher than the G0 and G100 groups (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). The G300 group also presented a higher <jats:styled-content style=\"fixed-case\">IEPS<\/jats:styled-content> proportion per number of viable cells compared to others. G300 presented greater surface roughness for both single\u2010 (mean roughness\u00a0=\u00a01460\u00a0\u03bcm) and dual\u2010species (mean roughness\u00a0=\u00a01990\u00a0\u03bcm) biofilms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Higher glucose concentration (300\u00a0mmol\/L) during biofilm development favors the growth of single\u2010 and dual\u2010species biofilms of <jats:italic>Candida<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jicd.12412","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T20:48:05Z","timestamp":1553719685000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Glucose supplementation effect on the acidogenicity, viability, and extracellular matrix of <i>Candida<\/i> single\u2010 and dual\u2010species biofilms"],"prefix":"10.1111","volume":"10","author":[{"given":"Natanael V. F.","family":"Bezerra","sequence":"first","affiliation":[{"name":"School of Dentistry Federal University of Para\u00edba Jo\u00e3o Pessoa Para\u00edba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arella C. M.","family":"Brito","sequence":"additional","affiliation":[{"name":"School of Dentistry Federal University of Para\u00edba Jo\u00e3o Pessoa Para\u00edba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana M. 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