{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:22:09Z","timestamp":1783426929504,"version":"3.54.6"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T00:00:00Z","timestamp":1560384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Invest &amp; Clin Dent"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>In the present study, we aimed to evaluate the cytosolic and nuclear\u2010mitochondrial expression of pro\u2010apoptotic and anti\u2010apoptotic markers in gingival tissue from patients with severe generalized chronic periodontitis (sGCP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010four patients participated in the study: 15 (54.3\u00a0+\u00a08.3\u00a0years) with sGCP and nine (38.2\u00a0+\u00a05.4\u00a0years) with gingivitis alone. Gingival tissue was collected using a 1.5\u2010mm diameter punch and homogenized using a cell disruptor. The supernatants were analyzed for the cytosolic and nuclear\u2010mitochondrial fractions of caspase\u20103, Bax, Bak, Smac, lamin B, Bad, Bim, survivin, Bcl\u2010xL, Mcl\u20101, and of the dimers Bcl\u20102\/Bax, Bcl\u2010xL\/Bak and Mcl\u20101\/Bak using a multiplex immunoassay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significantly higher levels of cytosolic Bcl\u2010xL\/Bak, nuclear\u2010mitochondrial Mcl\u20101\/Bak, and cytosolic Bcl\u2010xL were observed in gingival tissues from periodontitis patients compared to controls (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.03, 0.03, and 0.05, respectively). The patients with gingivitis presented significantly increased levels of nuclear\u2010mitochondrial Bad, cytosolic and nuclear\u2010mitochondrial Bcl\u20102\/Bax, and cytosolic Bim compared to the patients with periodontitis (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001, 0.03, 0.05, and 0.04, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Significantly higher levels of anti\u2010apoptotic markers, such as Bcl\u2010xL\/Bak, Mcl\u20101\/Bak, and Bcl\u2010xL, and lower levels of pro\u2010apoptotic markers Bad and Bim in inflamed tissues indicate an anti\u2010apoptotic trait in patients with sGCP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jicd.12422","type":"journal-article","created":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T10:59:03Z","timestamp":1560423543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Anti\u2010apoptotic traits in gingival tissue from patients with severe generalized chronic periodontitis"],"prefix":"10.1111","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0222-9231","authenticated-orcid":false,"given":"Carlos Marcelo","family":"Figueredo","sequence":"first","affiliation":[{"name":"Department of Periodontology, Faculty of Odontology Rio de Janeiro State University Rio de Janeiro Brazil"},{"name":"Department of Periodontology, Faculty of Dentistry and Oral Health Griffith University Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Cardoso","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Odontology Rio de Janeiro State University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane Fl\u00f4r Coelho","family":"de Souza Breves Beiler","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Odontology Rio de Janeiro 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